目的 对不同产地薏苡仁药材中的7种甘油三酯含量及2种不饱和脂肪酸含量进行测定，评价不同产地薏苡仁药材的质量。方法 采用HPLC-ELSD法测定薏苡仁药材中7种甘油三酯含量，建立HPLC-UV法测定油酸、亚油酸含量，并结合聚类分析对薏苡仁药材质量进行评价。结果 14批薏苡仁药材中三亚油酸甘油酯、1，2-二亚油酸-3-油酸甘油酯、1，2-二亚油酸-3-棕榈酸甘油酯、1，2-二油酸-3-亚油酸甘油酯、1-棕榈酸-2油酸-3-亚油酸甘油酯、三油酸甘油酯、1，2-二油酸-3-棕榈酸甘油酯质量分数分别在0.31%～0.83%、0.81%～1.81%、0.38%～0.95%、1.19%～2.39%、0.67%～1.58%、0.71%～1.55%、0.44%～1.13%；14批薏苡仁药材中油酸、亚油酸含量分别在0.45%～0.72%、0.37%～0.53%；聚类结果可知，以甘油三酯和脂肪酸为变量可以把薏苡仁药材聚为3类。结论 不同产地薏苡仁药材甘油三酯及脂肪酸的含量存在一定的差异，聚类结果未发现其含量和产地的相关性。多组分含量测定能更全面地反映薏苡仁药材的质量。

Objective To determine the seven kinds of triglycerides and two kinds of unsaturated fatty acids in Coicis Semen in order to evaluate the quality of Coicis Semen in different producing areas. Methods HPLC-ELSD method was used to determine the content of seven triglycerides in Coicis Semen. The HPLC-UV method was established to determine the content of oleic acid and linoleic acid. The cluster analysis method was used for quality evaluation. Results The content of trilinolein, 1,2-dilinoleic acid-3-olein, 1,2-dilinoleic acid-3-palmitin, 1,2-dioleic acid-3-linolein, 1-palmitic acid-2 oleic acid-3-linolein, triolein, 1,2-dioleic acid-3-palmitin were 0.31%-0.83%, 0.81%-1.81%, 0.38%-0.95%, 1.19%-2.39%, 0.67%-1.58%, 0.71%-1.55%, 0.44%-1.13%, respectively. The content of oleic acid and linoleic acid in 14 batches of Coicis Semen were 0.45%-0.72% and 0.37%-0.53%, respectively. Adopting triglyceride and fatty acid as variables, the samples can be grouped into three categories. Conclusion Some differences existed in content of triglyceride and fatty acid in Coicis Semen from different areas, but the clustering results did not find the correlation between its content and origin. The determination of multi-components comprehensively reflected the quality of Coicis Semen.

国家自然科学基金资助项目（81873016）；国家自然科学基金资助项目（81673606）；江苏省科教强卫医学重点人才项目（ZDRCA2016036）