目的 比较2种虎耳草科植物楔叶茶藨（RDP）和东北茶藨子（RMK）抗肾纤维化的药效作用差异。方法 采用体外实验与体内实验相结合的方法，利用转化生长因子-β1（TGF-β1）诱导的人肾小管上皮细胞（HK-2）纤维化模型和单侧输尿管结扎（UUO）诱导的小鼠肾纤维化模型，综合运用HE染色、Masson染色，免疫组化，免疫印迹等方法，以细胞形态、细胞长度与宽度比值，肾脏组织病理损伤程度，肾脏组织中E-钙黏蛋白（E-cadherin）和α-平滑肌肌动蛋白（α-SMA）的蛋白表达等为主要观测指标，比较RDP和RMK提取物对各项指标的影响；采用偏最小二乘-判别分析（PLS-DA）法，对RDP和RMK治疗组小鼠肾脏组织病理学评分进行分析。结果 RDP提取物（1.5、5、15μg/mL）与RMK提取物（3、10 μg/mL）均可以有效改善TGF-β1诱导的HK-2细胞形态改变，减小细胞长度与宽度比值；RDP（40 mg/kg）与RMK（80 mg/kg）均能显著减少UUO模型小鼠α-SMA表达和增加E-cadherin的蛋白表达，并减轻病理性损伤和纤维化程度；PLS-DA分析显示，RDP和RMK改善UUO诱导肾脏组织纤维化的作用无明显差异。结论 RDP和RMK体内外对肾纤维化均具有一定的保护作用，RMK和RDP对各项指标的改善作用无明显差别。

Objective To compare pharmacodynamic difference of Ribes diacanthum (RDP) and Ribes mandshuricum (RMK) treatment on renal fibrosis in vivo and in vitro. Methods Both of TGFβ1-induced HK-2 cell fibrosis model and UUO-induced kidney fibrosis mice model were used in the present study. The cell morphology, ratio of cell length to width, renal histopathology, protein expressions of α-SMA and E-cadherin in kidney tissues were evaluated through biological and pharmacological methods and technologies, including Western blot, immunohistochemistry, HE staining, Masson staining and so on. In addition, partial least squares-discriminant analysis (PLS-DA) was applied to analyze the renal histopathological score as well. Results RDP (1.5, 5, 15 μg/mL) and RMK (3, 10 μg/mL) effectively improved morphological changes and reduced the ratio of cell length to width in TGFβ1-induced HK-2 cell fibrosis; Moreover, RDP (40 mg/kg) and RMK (80 mg/kg) remarkably decreased the expression of α-SMA and increased the expression of E-cadherin in UUO mice model. The degree of pathological damage and fibrosis were also alleviated in both groups. PLS-DA analysis showed no significant difference in anti-fibrotic effects between RDP and RMK treatment. Conclusion Both RDP and RMK have anti-fibrosis effects on TGFβ1-induced HK-2 cell fibrosis model and UUO-induced kidney fibrosis mice model, and there is no significant difference between these two herbs.

楔叶茶藨肾保护活性物质基础及作用机制研究（81603389）；江苏省自然科学基金面上项目：基于转化医学研究楔叶茶藨肾保护作用的物质基础及作用机制研究（BK20160754）