目的 研究珠子参Panax japonicus var.major叶的化学成分。方法 采用硅胶、Sephadex LH-20、ODS柱色谱和半制备型高效液相色谱等方法进行分离纯化，根据理化性质和波谱数据分析鉴定化合物的结构。结果 从珠子参中分离并鉴定了3个化合物，分别鉴定为20（21），24-达玛二烯-3β，6α，12β-三醇（1）、20（22）Z，24-达玛二烯-3β，6α，12β-三醇（2）、3-O-[β-D-吡喃葡萄糖-（1→2）-β-D-吡喃葡萄糖]-20-O-[-β-D-吡喃木糖-（1→6）-β-D-吡喃葡萄糖]-23E，25-二烯-20（S）-原人参二醇（3）。结论 化合物1和2为首次从该植物中分离得到，化合物3为新化合物，命名为珠子参苷Z。

Objective To study the chemical composition from the leaves of Panax japonicus var. major. Methods Column chromatographies (including macroporous resin, silica gel, Sephadex LH-20 and ODS) and semi-preparative HPLC were used to separate the constituents. The structures were elucidated by the analysis of spectral data and chemical properties. Results Three compounds were isolated and elucidated as dammar-20(21),24-diene-3β,6α,12β-triol (1), dammar-20(22) Z,24-diene-3β,6α,12β-triol (2), and 3-O-[-β-D-glucopyranosyl-(1→2)-β-D-glucopyranosyl]-20-O-[-β-D-xylopyranosyl-(1→6)-β-D-glucopyranosyl]-23E,25-diene-20(S)-protopanoxadiol (3). Conclusion Compound 1 and 2 was obtained from the plant for the first time. And compound 3 named as majoroside Z is a new triterpenoid saponin.

陕西省教育厅服务地方专项计划项目（14JF005）；陕西省科技厅项目（2017JM8087）；陕西省科技厅项目（2018ZCXM-SF-007）；陕西中医药大学学科创新团队项目（2019-YL12）