目的 探究姜黄连炮制前后对大鼠能量代谢的影响差异，阐明姜制黄连药性的改变与能量代谢的关系。方法 分别制备生黄连、姜黄连水煎液，大鼠分别ig给予生黄连、姜黄连水煎液15 g/kg，每天1次，连续给药7 d，取大鼠血浆，检测糖酵解、三羧酸循环和氧化磷酸化3条能量代谢途径中关键酶的活性，并采用主成分分析法对检测结果进行分析，筛选出姜黄连炮制前后能量代谢发生主要变化的途径和关键酶。结果 与对照组比较，生黄连组己糖激酶（HK）、磷酸果糖激酶（PFK）、丙酮酸激酶（PK）及线粒体呼吸链复合体I、II、III、IV活性均显著下降，柠檬酸合酶（CS）、异柠檬酸脱氢酶（ICD）、α-酮戊二酸脱氢酶（α-KGDH）水平均显著下降；姜黄连组PFK、PK及线粒体呼吸链复合体II、III、IV的活性均显著降低，HK和线粒体呼吸链复合体I有一定程度下降，但差异不显著，α-KGDH水平显著降低，CS和ICD水平有一定程度降低，但差异不显著；与生黄连组比较，姜黄连组HK、PFK、PK及线粒体呼吸链复合体I、II、III活性均显著升高，CS、ICD、α-KGDH水平均显著升高，线粒体呼吸链复合体IV没有显著变化。通过主成分分析提取了2个主成分，其中氧化磷酸化途径中复合体II和复合体III分别对主成分2和主成分1有最大贡献率0.916、0.873。结论 黄连经姜制后寒凉之性减弱，可能是因为减弱了黄连对氧化磷酸化途径中线粒体呼吸链复合体II和III的抑制作用。

Objective To investigate the changes of energy metabolism before and after ginger-processing of Coptidis Rhizoma, and elucidate the relationship between the changes of medicinal properties and the energy metabolism. Methods Crude Coptidis Rhizoma and ginger-processed Coptidis Rhizoma decoctions were prepared and administered to rats once a day for 7 d by gavage. Blood and liver were used to detect the content and activity of key enzymes in three pathways of energy metabolism. The composition analysis analyzed the detection results and screened out the key enzymes for the main changes in energy metabolism before and after processing. Results Compared with the control group, the activities of HK, PFK, PK and mitochondrial respiratory chain complex I, II, III and IV in crude Coptidis Rhizoma group were decreased significantly, and the content of CS, ICD and α-KGDH was decreased significantly.The activities of mitochondrial respiratory chain complexes II, III and IV and the activities of PFK and PK in the ginger-processed Coptidis Rhizoma group were significantly decreased. HK and mitochondrial respiratory chain complex I was decreased to some extent, but there was no significant difference. The content of α-KGDH was decreased significantly, and the content of CS and ICD was decreased in a certain degree. Compared with the crude Coptidis Rhizoma group, the activities of HK, PFK, PK and mitochondrial respiratory chain complex I, II, and III in the ginger-processed Coptidis Rhizoma group were increased significantly, and the content of CS, ICD and α-KGDH was increased significantly. There was no significant change in the mitochondrial respiratory chain complex IV. Two principal components were extracted by principal component analysis. Among them, complex II and complex III had the maximum contribution rate of principal component 2 and principal component 1 of 0.916 and 0.873, respectively. Conclusion The weakening of cold in nature of Coptis chinensis after ginger-processed treatment may be due to the inhibition of the inhibitory effect of C. chinensis on mitochondria respiratory chain complex II and complex III in the oxidative phosphorylation pathway.

R285.5

国家科技基础性工作专项（2014FY111100-2）