目的 探讨盐补骨脂、盐小茴香组成的补骨脂丸和3种模拟补骨丸（盐补骨脂+生小茴香、生补骨脂+盐小茴香、生补骨脂+生小茴香）对脾肾阳虚泄泻大鼠的干预作用，从而确证药物盐炙组成补骨脂丸的合理性。方法 将SD大鼠随机分为9组，分别为对照组、模型组、阳性药参苓白术丸组、盐补骨脂组、生补骨脂组、补骨脂丸组及3种模拟补骨脂丸组。采用氢化可的松和番泻叶的复合造模法制备脾肾阳虚泄泻大鼠模型，于给药结束后测定各组大鼠的胸腺、脾脏、肾脏、肾上腺和睾丸脏器指数，观察相应脏器病理变化，并通过酶联免疫吸附法（ELISA）检测大鼠血清中胃动素和睾酮的水平。结果 与对照组相比，模型组大鼠摄食量减少，体质量减轻；胸腺、脾脏、肾脏、肾上腺和睾丸脏器指数均出现不同程度的下降；镜下观察到上述脏器均出现明显病变；胃动素水平呈上升趋势变化，睾酮水平显著降低。给予盐补骨脂、生补骨脂、补骨脂丸和3种模拟补骨脂丸后，上述症状和指标均得到不同程度的改善，且补骨脂丸的作用效果整体上优于补骨脂单用以及3种模拟补骨脂丸。结论 补骨脂丸温肾助阳、暖脾止泻的作用优于补骨脂单用，且盐炙后配伍使用止泻作用更佳。

Objective To investigate the effects of Buguzhi Pills and three kinds of simulative Buguzhi Pills on diarrhea rats with spleen-kidney yang deficiency, and to confirm the rationality of the salt-processed composition of Buguzhi Pills. Methods SD rats were randomly divided into nine groups:normal group, model group, positive drug group, salt-processed Psoraleae ructus group, raw Psoraleae Fructus group, Buguzhi Pills (salt-processed Psoraleae Fructus + salt-processed Foeniculi Fructus) group and three kinds of simulative Buguzhi Pills groups (salt-processed Psoraleae Fructus + raw Foeniculi Fructus; raw Psoraleae Fructus + salt-processed Foeniculi Fructus; raw Psoraleae Fructus + raw Foeniculi Fructus). A complex method of hydrocortisone and sennae folium was adopted to establish the diarrhea model due to spleen-kidney yang deficiency. After administration, the organ indexes of thymus, spleen, kidney, adrenal gland, and testis were measured; The pathological changes of corresponding organs were observed; The serum levels of motilin and testosterone were detected by ELISA. Results Compared with the normal group, the food intake and body weight reduced; The organ indexes decreased in varying degrees; The above organs showed obvious pathological changes under the microscope; the level of motilin showed an upward trend, and the level of testosterone decreased significantly in model group. The above symptoms and indicators were improved in varying degrees after administration, and the effect of Buguzhi Pills was better than that of Psoraleae Fructus alone and three kinds of simulative Buguzhi Pills on the whole. Conclusion The efficacy of Buguzhi Pills on warming kidney-spleen yang and relieving diarrhea was superior to that of Psoraleae Fructus alone, and the antidiarrheal effect can be enhanced by salt-processing.

国家自然科学基金面上项目（81873005）；河南省科技厅科技研发专项（192102310172）；河南省高等学校重点科研项目（19A360001）；河南省高等学校青年骨干教师培养计划（2018GGJS083）