目的 建立网果酸模Rumex chalepensis中6个成分的含量测定方法。方法 采用HPLC法，色谱柱Agilent Extend-C₁₈（250 mm×4.6 mm，5 μm），254 nm波长处二极管阵列检测器检测，流动相为甲醇-0.1%甲酸，梯度洗脱，体积流量1 mL/min，柱温25℃，进样量5 μL。结果 对照品大黄酚-8-O-β-D-葡萄糖苷、大黄素-8-β-D-吡喃葡萄糖苷、酸模素、大黄素、大黄酚、大黄素甲醚分别在208~3 120、22.40~336.35、178.9~2 908.8、16.7~250.8、104.4~1 566.0、45.2~677.7 ng，线性关系良好，平均回收率（n=6）分别为97.66%、97.10%、98.78%、97.38%、102.48%和95.51%。16批网果酸模中大黄酚-8-O-β-D-葡萄糖苷、大黄素-8-β-D-吡喃葡萄糖苷、酸模素、大黄素、大黄酚、大黄素甲醚的质量分数分别为0.6~7.1、0~2.7、1.0~6.5、0.1~0.6、0.7~4.3、0.1~0.4 mg/g；对比分析不同生长年限、不同采收期、不同地块样品含量，2年生网果酸模初春或夏末初秋采集，6个成分含量总和较高，为12.2 mg/g。结论 所建立的方法可用于同时测定网果酸模中6个成分含量，确定了网果酸模的采收年限和季节，为网果酸模药材质量评价标准的制定提供了科学依据。

Objective To establish a method for the determination of six components from Rumex chalepensis Mill.. Methods The contents of chrysophanol-8-O-β-D-glucoside, emodin-8-β-D-glucoside, nepodin, emodin, chrysophanol, and physcion were simultaneously determined by HPLC. The mobile phase was methanol-0.1% formic acid, gradient elution, flow rate of 1 mL/min, column temperature of 25℃, injection volume of 5 μL, detected by Agilent Extend-C₁₈ (250 mm×4.6 mm, 5 μm) and diode array detector at 254 nm wavelength. Results The content of chrysophanol-8-O-β-D-glucoside, emodin-8-β-D-glucoside, nepodin, emodin, chrysophanol, and physcionhad good linear relationship in the ranges of 208-3 120, 22.40-336.35, 178.9-2 908.8, 16.7-250.8, 104.4-1 566.0, 45.2-677.7 ng, respectively. The average recovery rates were 97.66%, 97.10%, 98.78%, 97.38%, 102.48%, and 95.51% (n=6). The contents of chrysophanol-8-O-β-D-glucoside, emodin-8-β-D-glucoside, nepodin, emodin, chrysophanol, and physcion in 16 batches of R. chalepensis. were determined in the range of 0.6-7.1, 0-2.7, 1.0-6.5, 0.1-0.6, 0.7-4.3, and 0.1-0.4 mg/g, respectively. Sample contents of different growing years, harvesting dates, and plots were compared and analyzed. Two-year-old R. chalepensis. was collected in early spring or late summer and early autumn. The total content of six components was 12.2 mg/g, which was relatively high. Conclusion The established method can be used for simultaneous determination of six components from R. chalepensis, and determine the harvesting time and season of R. chalepensis, which provides a scientific basis for the formulation of quality evaluation criteria of R. chalepensis.

中国中医科学院基本科研业务费自主选题项目（YZ-1721）；湖北省卫生健康委员会2018—2019年度中医药科研立项项目（鄂卫通[2019]13号-ZY2019F015）；荆州市2018年度科技发展计划项目（荆科技发[2018]37号-2018091）