目的 优化番石榴叶总黄酮提取工艺。方法 以乙醇体积分数、料液比、提取时间、提取次数4个因素为考察对象，进行L₉（3⁴）4因素3水平正交设计，以信息熵赋权法计算总黄酮得率及提取物中金丝桃苷、槲皮素-3-O-β-D-吡喃阿拉伯糖苷、槲皮素-3-O-α-L-呋喃阿拉伯糖苷得率4个评价指标权重系数并进行加权处理得综合评分，采用综合评分进行正交分析筛选最佳工艺。结果 番石榴叶总黄酮的最佳提取工艺为以8倍量的70%乙醇提取3次，每次2 h。3批验证实验综合评分均值为0.142 1，RSD为2.37%。结论 优化的番石榴叶总黄酮的提取工艺稳定可行，得率高。

Objective To optimize the extraction of total flavonoids from leaves of Psidium guajava. Methods L₉(3⁴) orthogonal test was used to screen the optimal extraction technology of total flavonoids from leaves of P. guajava with ethanol concentration, solid-liquid ratio, extraction time, and extraction times as factors. The weight coefficient of the four evaluation indicators, including the yield of total flavonoids, hyperoside, quercetin-3-O-β-D-pyranarabinoside, and quercetin-3-O-α-L-furanarabinoside was calculated by information entropy weighting so as to calculate comprehensive score. We obtained the optimal technology by orthogonal analysis based on the comprehensive score. Results The optimal extraction technology was that using 8-fold 70% ethanol water to extract 2 h for three times. The mean comprehensive score of the three batches was 0.142 1 and the RSD was 2.37%. Conclusion The optimal extraction technology of total flavonoids from leaves of P. guajava was stable and feasible with high yield.

国家科技重大专项重大新药创制项目（2017ZX09301062）；天津市卫生局科技基金攻关课题（14KG131）；天津市卫健委、天津市中医药管理局中医、中西医结合科研基金项目（2019039）；天津市中医药管理局中医、中西医结合科研基金项目（2015024）