目的 优化栀子炭炮制工艺，并结合药效学研究对所得炮制工艺进行验证。方法 运用热分析技术分别对栀子中不同有效成分进行热解特性分析，得出栀子炭最佳炮制温度范围；同时，以栀子炭中鞣质含量为指标，选择炒制温度、炒制时间2个因素，结合响应面法试验优化栀子炭炮制工艺，并通过药效学实验（止血实验）对栀子炭炮制工艺进行验证。结果 热分析实验综合分析得出栀子炭最佳炮制温度为290.3~387.0℃；响应面法优化得出栀子炭最优炮制工艺为炒制温度330℃，炒制时间5.91 min；与空白组比较，新法组、传统组均能极显著缩短小鼠凝血时间（P<0.01），生品组能显著缩短小鼠凝血时间（P<0.05）；新法组能显著缩短小鼠活化部分凝血活酶时间（APTT）（P<0.05），对小鼠凝血酶原时间（PT）、凝血酶时间（TT）的影响差异不显著。结论 结合热分析技术和响应面法优化栀子炭炮制工艺，并进行相关药效学实验验证，结果合理可行，该法可为改进传统中药炮制工艺，合理量化炮制过程中火力火候参数提供借鉴。

Objective To optimize the processing technology of Gardenia jasminoides carbon and validate the process with pharmacodynamics research. Methods The pyrolysis characteristics of different active components in G. jasminoides were analyzed by thermal analysis technology, and the optimum processing temperature range of G. jasminoides carbon was obtained; Taking the tannin content in G. jasminoides carbon as the index, two factors of stir-frying temperature and stir-frying time was chosen to optimize the processing technology of G. jasminoides carbon by response surface methodology, and the processing technology of G. jasminoides carbon was verified by pharmacodynamics experiment (hemostasis test). Results The optimum processing temperature range of G. jasminoides carbon was 290.3-387℃, response surface methodology was used to optimize the processing technology of G. jasminoides carbon:The processing temperature was 330℃, the processing time was 5.91 min, and the tannin content was 3.120 mg/g; Compared with the blank group, the new method group and the traditional group could significantly shorten the clotting time of mice (P < 0.01), raw product group can significantly shorten the clotting time of mice (P < 0.05), the new method group can significantly shorten APTT in mice (P < 0.05), there was no significant difference in PT and TT in mice. Conclusion The processing technology of G. jasminoides carbon was optimized by thermal analysis technology and response surface methodology, and the results were verified by pharmacodynamics experiments. The method can provide a reference for improving the processing technology of Chinese materia medica and quantifying the fire parameters in the process of processing.

山西省科技厅重点研发计划重点项目：12种优质中药饮片规范化炮制加工工艺及质量标准研究（201603D3112002）