目的 基于新型核壳色谱技术建立补阳还五汤（BHD）的指纹图谱，为其质量控制提供依据。方法 采用Agilent Poroshell 120 SB-C₁₈（150 mm×4.6 mm，2.7 μm）色谱柱，以乙腈-0.1%甲酸水溶液为流动相，体积流量为0.8 mL/min，柱温30℃，检测波长为280 nm。采用"中药色谱指纹图谱相似度评价系统2012版"对15批BHD指纹图谱进行评价分析。采用对照品比对和高分辨质谱技术鉴定了21个化合物。结果 在38 min内建立了BHD的指纹图谱，共确定了21个共有峰（P1~P21），涵盖了组方中全部7味药材。同时采用四级杆-静电场轨道阱高分辨质谱（Q-Orbitrap HRMS/MS）、对照品及阴性方比较法对21个共有峰进行了归属鉴定，其中奎宁酸甲酯（P4）、毛蕊异黄酮苷（P11）、毛蕊异黄酮苷-6"-O-丙二酸酯（P13）、芒柄花苷（P15）、（6αR，11αR）-9，10-二甲氧基紫檀烷-3-O-β-D-葡萄糖苷（P16）、异微凸剑叶莎醇-7-O-β-D-葡萄糖苷（P17）、毛蕊异黄酮（P18）、芒柄花素（P19）、（6αR，11αR）-9，10-二甲氧基紫檀烷（P20）、异微凸剑叶莎醇（P21）来源于黄芪；琥珀酰基腺苷（P3）、6-羟基山柰酚-3，6-O-二葡萄糖-7-O-葡萄糖醛酸苷（P5）、山柰酚-3-O-槐糖苷（P10）来源于红花；没食子酸（P2）、芍药苷（P9）来源于赤芍；香草酸（P7）来源于当归；鸟苷（P1）来源于地龙；苦杏仁苷（P6）、野樱苷（P8）来源于桃仁；阿魏酸（P12）、洋川芎内酯H（P14）来源于当归和川芎。结论 首次采用核壳色谱技术建立了BHD的UHPLC指纹图谱，方法简便、耗时短、专属性强，并结合共有峰的高分辨质谱定性分析，为BHD的质量评价及药效物质基础研究奠定了基础。

Objective To establish the fingerprint of Buyang Huanwu Decoction (BHD) based on core-shell column for its quality control. Methods The extract of BHD was separated on an Agilent Poroshell 120 SB-C₁₈ (150 mm×4.6 mm, 2.7 μm) with a gradient elution. The mobile phase consisted of acetonitrile-0.1% formic acid. The detection wavelength was 280 nm. The similarity evaluation of 15 batches of BHD was carried out by the "Similarity Evaluation System for Chromatographic Fingerprints of TCM". HPLC-Q-Orbitrap HRMS/MS was used to characterize the 21 chemical compounds of the extract of BHD. Results The chromatographic fingerprints of 15 batches of BHD were generated 21 common peaks (P1-P21), belonging to all seven medicinal herbs in 38 min. Methyl quinate (P4), calycosin-7-O-β-D-glucoside (P11), calycosin-7-O-β-D-glucoside-6"-O-malonate (P13), ononin (P15), (6αR, 11αR)-9,10-dimethoxypterocarpan-3-O-β-D-glucoside (P16), isomucronulatol-7-O-β-D-glucoside (P17), calycosin (P18), formononetin (P19), (6αR,11αR)-9,10-dimethoxypterocarpan (P20), and isomucronulatol (P21) were from Astragali Radix. Succinyl adenosine (P3), 6-hydroxykaempferol-3,6-diglucoside (P5), and kaempferol-3-O-sophoroside (P10) were from Carthami Flos. Gallic acid (P2) and paeoniflorin (P9) were from Paeoniae Radix Rubra. Vanillic acid (P7) was from Angelicae Sinensis Radix. Guanoside (P1) was from Pheretima. Amygdalin (P6) and prunasin (P8) were from Persicae Semen. Ferulic acid (P12) and senkyunolide H (P14) were from Angelicae Sinensis Radix and Chuanxiong Rhizoma. A total of 21 components were identified by UHPLC-Q-Orbitrap HRMS/MS. It was the first time to establish the UHPLC fingerprint of BHD by core-shell chromatography technology. Conclusion The method is simple and accurate with a good reproducibility and time consuming, which will provide a basis for the further research on effective constituents in BHD.

国家自然科学基金项目（81703671）；国家自然科学基金项目（81773884）；广东省科技计划项目（2017A020213029）；广东省中医药局科研项目（20181066）；广东省联合培养研究生示范基地项目；2017年中央支持地方高校发展专项资金项目（众创空间建设）