目的 研究白花败酱草Patrinia villosa的化学成分及其抗氧化活性。方法 利用硅胶柱色谱、ODS柱色谱、葡聚糖凝胶柱色谱法进行分离，应用半制备高效液相色谱进行进一步的分离纯化，通过核磁共振技术等波谱数据鉴定化合物结构。采用DPPH法和ABTS法评价各化合物的体外抗氧化活性。结果 从白花败酱草70%乙醇提物中分离得到10个化合物，分别为绿原酸丁酯（1）、3，4-O-双咖啡酰奎尼酸甲酯（2）、木犀草素-7-O-芸香苷（3）、1β-O-β-D-glucopyranosy-15-O-（p-hydroxylphenylacetate）-5α，6βH-eudesma-3，11（13）-dien-12，6α-olide（4）、3，4-O-双咖啡酰奎尼酸乙酯（5）、4，5-O-双咖啡酰奎尼酸甲酯（6）、4，5-O-双咖啡酰奎尼酸正丁酯（7）、木犀草素-7-O-β-D-葡糖醛酸苷甲酯（8）、木犀草素-7-O-β-D-葡糖醛酸苷乙酯（9）、芹菜素-7-O-β-D-葡糖醛酸苷甲酯（10）。化合物3、8、9的清除DPPH自由基IC₅₀分别为（23.95±0.71）、（73.09±0.33）、（25.06±0.65）μmol/L，其清除ABTS自由基IC₅₀分别为（7.13±0.07）、（11.48±0.21）、（5.15±0.08）μmol/L。结论 除化合物3、8外，其余化合物均为首次从白花败酱草中分离得到。化合物3、8、9具有明确的体外抗氧化活性。

Objective To study the antioxidant chemical constituents and antioxidant activity of Patrinia villosa. Methods The 70% ethanol-water extract of the herb was separated by silica gel column chromatography, ODS column chromatography and sephadex column chromatography. Then, the compound were further purified and extracted by semi-preparative HPLC. Their structures were elucidated by physiochemical property and spectral analysis. DPPH and ABTS methods were used to determine the antioxidant bioactivities of the isolated compounds. Results A total of ten compounds were isolated and synthesized, including chlorogenic acid butyl ester (1), 3,4-di-O-caffeoyl quinic acid methyl ester (2), luteolin-7-O-rutinoside (3), 1β-O-β-D-glucopyranosy-15-O-(p-hydroxylphenylacetate)-5α,6βH-eudesma-3,11(13)-dien-12,6α-olide (4), 3,4-di-O-caffeoyl quinic acid ethyl ester (5), 4,5-di-O-caffeoyl quinic acid methyl ester (6), 4,5-di-O-caffeoyl quinic acid n-butyl ester (7), luteolin-7-O-β-D-glucuronide methyl ester (8), luteolin-7-O-β-D-glucuronide ethyl ester (9), and apigenin-7-O-β-D-glucuronide methyl ester (10). The DPPH radical scavenging IC₅₀ of compounds 3, 8, and 9 were (23.95 ±0.71), (73.09 ±0.33), and (25.06 ±0.65) μmol/L, respectively. The ABTS radical scavenging IC₅₀ was (7.13 ±0.07), (11.48 ±0.21), (5.15 ±0.08) mol/L, respectively. Conclusion Eight compounds except compounds 3 and 8 are obtained from this species for the first time. Compounds 3, 8, and 9 had significant antioxidant activity.

国家自然科学基金项目（81303211）；中国博士后科学基金项目（2013M540301）；黑龙江省普通本科高等学校青年创新人才培养计划（UNPYSCT-2017201）