[关键词]
[摘要]
目的 研究甘草Glycyrrhizae Radix et Rhizoma黄酮类化学成分。方法 采用HP-20大孔树脂、硅胶、ODS、凝胶等多种柱色谱，结合制备液相等方法进行分离和纯化，根据理化性质及波谱数据对化学成分进行结构鉴定。结果 从甘草70%乙醇提取物中分离得到10个黄酮类化合物，分别鉴定为4'，6，7-三羟基-2'-甲氧基查耳酮（1）、3'，4'，5，7-四羟基-8-（3-羟基-3-甲基丁基）-异黄酮（2）、异甘草素（3）、异甘草苷（4）、刺甘草查耳酮（5）香豌豆酚（6）、芒柄花苷（7）、2（S）-3'，5'，7-三羟基二氢黄酮（8）、南酸枣苷（9）、4'，7-二羟基黄酮（10）。结论 化合物1和2为新化合物，分别命名为异甘草查耳酮B和甘草异黄酮G，化合物9为首次从该属植物中分离得到。
[Key word]
[Abstract]
Objective To study the chemical constituents of flavonoids from Glycyrrhizae Radix et Rhizoma. Methods The compounds were isolated and purified by column chromatography over HP-20 macroporous resin, silica gel, Sephadex LH-20, and preparative RP-HPLC. Their structures were elucidated by physicochemical properties and spectral analyses. Results Ten flavonoids were isolated and identified as 4',6,7-trihydroxy-2'-methoxyl-chalcone (1), 3',4',5,7-tetrahydroxy-8-(3-hydroxy-3-methylbutyl)-isoflavone (2), isoliquiritigenin (3), isoliquiritin (4), echinatin (5), orobol (6), ononin (7), 2(S)-3',5',7-trihydroxy-flavanone (8), 2(S)-naringenin-4'-O-β-D-glucopyranoside (9), and 4',7-dihydroxyflavone (10). Conclusion Compounds 1 and 2 are new compounds named isolicochalcone B and licoisoflavone G, while compound 9 is isolated from the genus for the first time.
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[基金项目]
广东省科技计划项目（2015B030301005）
