目的 研究川芎内生真菌Alternaria sp.IS275代谢产物的化学成分。方法 采用硅胶和葡聚糖凝胶Sephadex LH-20柱色谱、反相中压液相色谱、制备薄层色谱以及半制备高效液相色谱等分离技术，对川芎内生真菌发酵液的醋酸乙酯提取部位进行分离纯化，利用现代波谱学手段对分离的化学成分进行结构鉴定。结果 从川芎内生真菌Alternaria sp.IS275代谢产物的醋酸乙酯提取部位分离得到1个新的内酯类化合物，鉴定为（+）-4，11-二羟基-2-十二烯-5-内酯。结论 化合物1为首次从川芎内真菌中分离得到的新内酯化合物，命名为芎孢内酯A。

Objective To study the chemical constituents of metabolites of Alternaria sp. IS275, an endophytic fungus isolated from Ligusticum chuanxiong. Methods The extraction fraction of ethyl acetate from endophytic fungi fermentation broth of L. chuanxiong were isolated and purified by silica gel, Sephadex LH-20, reversed phase medium pressure chromatography, preparative TLC, and semi-preparative RP-HPLC. The structure of the separated chemical constituent was elucidated by modern spectroscopy technology. Results A new lactone compound (+)-4,11-dihydroxy-2-dodecene-5-lactone was isolated from the ethyl acetate fraction of the metabolite of Alternaria sp. IS275, an endophytic fungus of L. chuanxiong. Conclusion Compound 1 is a new lactone compound, named ligualterlide A.

四川省川产道地药材综合开发与区域发展项目（2017ZY002）；四川省科技创新苗子工程培育项目（2018086，2019046）；四川省青年科技创新研究团队专项计划项目（2017TD0001，2016TD0006）