目的 采用UPLC-Q-TOF-MS对止动颗粒血浆及尿液中移行成分进行快速定性，为体内药效物质基础研究提供参考。方法 Waters UPLC HSS T3（150 mm×2.1 mm，1.8 μm）色谱柱，0.1%甲酸水（A）-乙腈（B）梯度洗脱，体积流量0.3 mL/min，柱温35℃，采用电喷雾电离化源（ESI），MS^E扫描模式进行正、负离子检测。结果 通过与空白生物样品和对照品的提取离子色谱图、质谱碎片裂解信息比对，初步鉴定出45个血浆及尿液中移行成分，包括28个原型成分和17个代谢产物。结论 建立的UPLC-Q-TOF-MS分析方法可为止动颗粒在血浆及尿液中移行成分的定性分析提供一种快速、简便、可靠的分析手段，为该制剂的药效物质基础研究奠定基础。

Objective To investigate the prototype components and metabolites of Zhidong Granule in rats plasma and urine by UPLC-Q-TOF-MS. Methods The analysis was carried out on a Waters UPLC HSS T3 (2.1 mm×150 mm, 1.8 μm) column, with the mobile phase of 0.1% formic acid solution (A)-acetonitrile (B) at a flowing rate of 0.3 mL/min, the column temperature was 35℃. Electrospray ionization (ESI) source was applied and operated in both positive and negative ion mode. Results A total of 45 components, including 28 prototype components and 17 metabolites, were identified by comparing their retention time, ion fragmentation information with that of the blank biological samples, herb extract, and reference compounds. Conclusion This experimental method is simple and reliable, which can provide theoretical basis for elucidating the bioactive components of Zhidong Granule.

国家科技重大专项重大新药创制项目-面向国际的创新中药大平台建设（2013ZX09402202）