目的 建立一测多评法同时测定竹节参中7种皂苷类成分的含量，并验证此方法在竹节参中应用的可行性与适用性。方法 采用HPLC法，以竹节参皂苷Ⅴ为内参物，建立人参皂苷Rg₁、Re、Rb₁，假人参皂苷RT₁，竹节参皂苷Ⅳ、Ⅳa的相对校正因子（f_s/i），并利用f_s/i计算竹节参样品中成分的含量，同时用外标法测定各成分的含量，比较2种方法的差异。结果 人参皂苷Rg₁、Re、Rb₁，假人参皂苷RT₁，竹节参皂苷Ⅳ和Ⅳa的f_s/i分别为1.286 7、1.432 7、0.966 6、0.962 4、1.207 2、0.938 4。采用一测多评法与外标法得到的10批竹节参样品各成分含量无显著性差异。结论 建立的一测多评法准确性高，可用于竹节参皂苷类成分的定量分析和质量评价。

Objective To establish quantitative analysis of multi-components by single marker (QAMS) method for simultaneous determining the content of seven saponins in Panacis Japonici Rhizoma, and to evaluate the adaptation and application of QAMS method in the quality control of Panacis Japonici Rhizoma. Methods The relative factor (f_s/i) of ginsenoside Rg₁, Re, Rb₁, pseudoginsenoside RT₁, chikusetsusaponin IV and Ⅳa were established by HPLC method with chikusetsusaponin V as internal standard, which were used to calculate the content of seven saponins in Panacis Japonici Rhizoma. Meanwhile, external standard method (ESM) was used to calculate the content of seven saponins. The difference between QAMS and ESM was analyzed to evaluate the accuracy of QAMS. Results The f_s/i of ginsenoside Rg₁, Re, Rb₁, pseudoginsenoside RT₁, chikusetsusaponin IV and IVa were 1.286 7, 1.432 7, 0.966 6, 0.962 4, 1.207 2, and 0.938 4. There was no significant difference between the content of 10 batches of Panacis Japonici Rhizoma determined by QAMS and ESM. Conclusion The established QAMS method is accurate and can be used for quantitative analysis and quality evaluation of the content of seven saponins in Panacis Japonici Rhizoma.

湖南省重点研发计划项目（2017SK2123）；湖南省中医药科研计划重点项目（201811）；长沙市科技计划重点项目（kq1801034）；湖南中医药大学一层次学科中药学资助（湘教通[2011]76）