目的 探讨植物的形态特征及ITS2、psbA-trnH序列在薄荷及其近缘物种鉴定中的应用，为薄荷及近缘物种的鉴定提供参考。方法 观察薄荷及其近缘物种叶片形状、叶缘形状、绒毛密度、腺鳞密度等形态特征并对供试材料ITS2序列、psbA-trnH序列进行PCR并测序，通过对比植物形态特征、分析DNA序列、计算遗传距离、构建邻接树等方法对数据进行整理分析，并对植物进行鉴定。结果 植物的形态特征结合ITS2序列、psbA-trnH序列构建的邻接树显示香水薄荷与夏香薄荷、丹参与紫苏、胡椒薄荷与美国薄荷、大叶薄荷与猫薄荷亲缘关系近，该方法能将薄荷及其近缘物种区分。结论 ITS2序列、psbA-trnH序列可作为薄荷及其近缘物种的DNA条形码，与叶的形态特征相结合鉴定效果更佳。

Objective The morphological characteristics of plants and the application of ITS2 and psbA-trnH sequences in the identification of Mentha haplocalyx and its related species were discussed in order to provide references for the identification of M. haplocalyx and its related species. Methods The morphological characteristics such as the shape of leaf and leaf edge, villous density and glandular scale density of M. haplocalyx and its related species were observed. Alignment was performed to compare ITS2 and psbA-trnH sequences of the tested materials. The data were processed and analyzed by means of comparing the morphological characteristics of plants, analyzing the DNA barcode sequences, calculating the genetic distance, and constructing NJ trees. Results The NJ trees were constructed according to the morphological characteristics of plants combined with ITS2 and psbA-trnH sequences, which showed that M. arvensis was closely related to Satureja Hortensis, Salvia miltiorrhiza was closely related to Perilla frutescens, Mentha piperita was closely related to the Monarda didyma and Agastache rugosa was closely related to Nepeta cataria. This method could distinguish M. haplocalyx from its related species. Conclusion ITS2 and psbA-trnH sequences can be used as DNA barcode of M. haplocalyx and its related species, and the combination with morphological characters of leaves is better for the identification.

河北中医药管理局科研计划项目（2015003）；河北省高等学校科学技术研究项目（BJ2016039）；河北中医学院博士科研基金资助项目（BSZ2016002）；河北省二期现代农业产业技术体系创新团队项目（HBCT2018060205）