温馨提示：建议您使用Chrome80、火狐74+、IE9+浏览器 ，当您的浏览器版本过低，可能会影响部分功能正常使用。

主办：天津药物研究院 中国药学会

微信公众号

网站二维码

2025年6月13日 12:04 星期五
首页 > 过刊浏览>2019年第50卷第15期 >2019,50(15):3660-3666. DOI:10.7501/j.issn.0253-2670.2019.15.022
上一篇 | 下一篇

红花CtWD40基因克隆及表达分析

Clone and expression analysis of CtWD40 in Carthamus tinctorius

发布日期：2019-08-12
  • 摘要
  • 访问统计
  • PDF预览
  • 参考文献
  • 相似文献
    [关键词]
    [摘要]
    目的 克隆红花WD40（CtWD40）转录因子，分析其在不同组织间的表达水平及与红花羟基黄色素含量的相关性。方法 以红花花瓣转录组WD40候选基因为参考，克隆获得CtWD40基因序列。同时对该转录因子保守结构域、蛋白三维结构及系统发育等内容进行生物信息学分析，荧光定量PCR方法研究了该基因在红花不同组织中基因表达差异，并采用HPLC法测定红花各花期花瓣中羟基红花黄色素A含量。结果 成功克隆CtWD40基因并且发现该蛋白序列中8个保守WD结构域，通过系统发育分析发现CtWD40与菊科WD40蛋白亲缘关系最近。结论 CtWD40基因在在不同花期花瓣组织中出现先升高后降低表达趋势，皮尔森相关系数分析揭示CtWD40在花瓣中基因表达水平与羟基黄花黄色素A含量具有显著相关性。
    [Key word]
    [Abstract]
    Objective To clone CtWD40 transcription factors (TFs) and analyze its expression level in different tissues of Carthamus tinctorius and relationship with the content of hydroxysafflor yellow A (HSYA). Methods CtWD40 gene was obtained by cloning the WD40 candidate gene from transcriptome of C. tinctorius as reference. Its conserved domain, three-dimensional structure and phylogeny analysis were analyzed by bioinformatics methods. The CtWD40 expression pattern was also analyzed by qRT-PCR method, in the same time, HSYA content in different petals were analyzed by HPLC. Results Gene sequence of CtWD40 was obtained and eight conserved WD domains were found in CtWD40 gene sequences. Phylogenetic analysis revealed that CtWD40 had a closed homology with WD40 from composite plants. Conclusion The expression of CtWD40 gene was first increased and then decreased in petal tissues of different flowering stages.Pearson coefficient revealed significant correlation between CtWD40 expression and HSYA content in C. tinctorius petal.
    [基金项目]
    国家自然科学基金资助项目（31501366）；吉林省科技厅项目（20170520089JH）；吉林省教育厅项目（JJKH20190918KJ）
    [1] Smith T F, Gaitatzes C, Saxena K. The WD repeat:A common architecture for diverse functions[J]. Trends Biochem Sci, 1999, 24(5):181-185.
    [2] Angers S, Li T, Yi X. Molecular architecture and assembly of the DDB1-CUL4A ubiquitin ligase machinery[J]. Nature, 2006, 443(7111):590-593.
    [3] Jin J, Arias E E, Chen J. A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2, which is required for S phase destruction of the replication factor Cdt1[J]. Mol Cell, 2006, 23(5):709-721.
    [4] Wakasugi M, Kawashima A, Morioka H. DDB accumulates at DNA damage sites immediately after UV irradiation and directly stimulates nucleotide excision repair[J]. J Biol Chem, 2002, 277(3):1637-1640.
    [5] Ajuh P, Sleeman J, Chusainow J. A direct interaction between the carboxyl-terminal region of CDC5L and the WD40 domain of PLRG1 is essential for pre-mRNA splicing[J]. J Biol Chem, 2001, 276(45):42370-42381.
    [6] Higa L A, Wu M, Ye T. CUL4-DDB1 ubiquitin ligase interacts with multiple WD40-repeat proteins and regulates histone methylation[J]. Nat Cell Biol, 2006, 8(11):1277-1283.
    [7] Chen R H, Miettinen P J, Maruoka E M. A WD-domain protein that is associated with and phosphorylated by the type Ⅱ TGF-beta receptor[J]. Nature, 1995, 377(6549):548-552.
    [8] Van N S, Ludwig P. The WD-repeat protein superfamily in Arabidopsis:Conservation and divergence in structure and function[J]. BMC Genomics, 2003, 4(1):50-54.
    [9] Mishra A K, Muthamilarasan M, Khan Y. Genome-wide investigation and expression analyses of WD40 protein family in the model plant foxtail millet (Setaria italica L.)[J]. PLoS One, 2014, 9(1):e86852.
    [10] Ouyang Y, Huang X, Lu Z. Genomic survey, expression profile and co-expression network analysis of OsWD40 family in rice[J]. BMC Genomics, 2012, 13:100-105.
    [11] Zhu Y, Huang S, Miao M. Genome-wide identification, sequence characterization, and protein-protein interaction properties of DDB1(damaged DNA binding protein-1)-binding WD40-repeat family members in Solanum lycopersicum[J]. Planta, 2015, 241(6):1337-1350.
    [12] Burchill L, Velkoska E, Dean R G. Acute kidney injury in the rat causes cardiac remodelling and increases angiotensin-converting enzyme 2 expression[J]. Exp Physiol, 2008, 93(5):622-630.
    [13] Liu W C, Zheng S Q, Yu Z D. WD40-REPEAT 5a represses root meristem growth by suppressing auxin synthesis through changes of nitric oxide accumulation in Arabidopsis[J]. Plant J, 93(5):883-893.
    [14] Kong D, Li M, Dong Z. Identification of TaWD40D, a wheat WD40 repeat-containing protein that is associated with plant tolerance to abiotic stresses[J]. Plant Cell Rep, 2015, 34(3):395-410.
    [15] Starkevic P, Paukstyte J, Kazanaviciute V. Expression and anthocyanin biosynthesis-modulating potential of sweet cherry (Prunus avium L.) MYB10 and bHLH genes[J]. PLoS One, 2015, 10(5):e0126991.
    [16] Aguilar B. Virus-induced silencing of MYB and WD40 transcription factor genes affects the accumulation of anthocyanins in chilli pepper fruit[J]. Biol Plant, 2014, 58(1):567-574.
    [17] Zhu Q, Sui S, Lei X. Ectopic expression of the coleus R2R3 MYB-Type proanthocyanidin regulator gene SsMYB3 alters the flower color in transgenic tobacco[J]. PLoS One, 2015, 10(10):e0139392.
    [18] Li Y, Shan X, Gao R. Two Ⅲf Clade-bHLHs from Freesia hybrida play divergent roles in flavonoid biosynthesis and trichome formation when ectopically expressed in Arabidopsis[J]. Sci Rep, 2016, 6(1):30514.
    [19] Li H, Dong Y, Yang J. De novo transcriptome of safflower and the identification of putative genes for oleosin and the biosynthesis of flavonoids[J]. PLoS One, 2012, 7(2):e30987.
    [20] Salih H, Gong W, Du X M. Genome-wide characterization, identification and expression analysis of WD40 proteins family in cotton[J]. Genome, 2018, 61(7):539-547.
    [21] Bian S, Li X, Mainali H. Genome-wide analysis of DWD proteins in soybean (Glycine max):Significance of Gm08DWD and GmMYB176 interaction in isoflavonoid biosynthesis[J]. PLoS One, 2017, 12(6):e0178947.
    [22] Liu Y, Zhou B, Qi Y. Expression differences of pigment structural genes and transcription factors explain flesh coloration in three contrasting kiwifruit cultivars[J]. Front Plant Sci, 2017, 8(1):1507-1513.
    [23] Mateus A R, Marques P M, Oostra V. Adaptive developmental plasticity:Compartmentalized responses to environmental cues and to corresponding internal signals provide phenotypic flexibility[J]. BMC Biol, 2014, 12(1):97-102.
友情链接中华人民共和国科学技术部
您是第12393861位访问者
中草药 ® 2025 版权所有
技术支持：北京勤云科技发展有限公司
津备案：津ICP备13000267号 互联网药品信息服务资格证书编号：（津）-非经营性-2015-0031
请使用 Firefox、Chrome、IE10、IE11、360极速模式、搜狗极速模式、QQ极速模式等浏览器，其他浏览器不建议使用!