目的 探讨山茱萸对视黄醇转运的调控作用，阐述其治疗干眼症的作用机制，丰富"酸补肝明目"的分子基础。方法 60只BALB/c小鼠随机分为6组，每组10只，分别为对照组、模型组、阳性对照（石斛夜光丸2.9 g/kg）组及山茱萸低、中、高剂量（12、24、48 g/kg）组。采用苯扎氯铵滴眼法诱导干眼症模型小鼠，ig给予不同剂量的山茱萸水煎液，通过检测各组小鼠泪液分泌量、泪膜破裂时间，评价山茱萸水煎剂对干眼症模型小鼠的干预作用。ELISA法检测血清前蛋白（PA）、视黄醇结合蛋白（RBP）水平、肝脏中视黄醇水平；免疫组织化学法（IHC）及Western blotting法检测泪腺组织中胞内视黄醇结合蛋白（CRBP）及RBP受体（STRA6）的表达水平。结果 与对照组比较，模型组小鼠泪液分泌量显著减少，泪膜破裂时间显著缩短（P<0.01），泪腺组织中CRBP及STRA6蛋白表达水平显著降低（P<0.01）。与模型组比较，各给药组小鼠血清PA、RBP及肝脏组织中视黄醇水平显著升高（P<0.01），泪腺组织中CRBP及STRA6蛋白表达水平显著升高（P<0.05、0.01）。结论 干眼症发生时，机体伴随视黄醇缺乏和转运障碍，而酸味药山茱萸水煎液可以促进视黄醇吸收，改善机体视黄醇缺乏状态，进而调控其转运过程，这可能是"酸入肝，明目"的分子机制之一。

Objective To investigate the regulatory effect of Corni Fructus on retinol transport, and to elucidate its molecular mechanism in the treatment of dry eye syndrome and enrich the basis of "acid tonifying liver and improving vision". Methods A total of 60 BALB/c mice were randomly divided into six groups with 10 mice in each group, including control group, model group (saline 0.02 mL/g), positive control group (Dendrobium Nocturnal Pills 2.9 g/kg), Corni Fructus low, medium, and high dose group (12, 24, and 48 g/kg). The dry eye model mice were induced by the benzalkonium chloride eye drops. After successful modeling, mice were treated with different doses of Corni Fructus decoction for four consecutive weeks. One hour after the last dose, the intervention of Corni Fructus decoction on dry eye model mice was evaluated by determining the amount of tear secretion and tear film rupture time. The content of serum preprotein (PA), RBP, and retinol in liver was detected by ELISA method. The expression of CRBP and STRA6 in lacrimal gland was detected by IHC and Western blotting. Results Compared with the control group, the tear secretion and tear film rupture time in the model group were significantly decreased, and the serum PA, RBP, and the content of vitamin A in the liver of the other groups were significantly lower than those of the control group (P < 0.01). Compared with the model group, the levels of serum PA, RBP, and vitamin A in liver were significantly increased in each drug intervention group (P < 0.01). Compared with the control group, the content of CRBP and STRA6 in the lacrimal gland of the model group decreased significantly (P < 0.01). Compared with the model group, the content of CRBP and STRA6 in the lacrimal gland of each drug intervention group was significantly higher than those in the model group (P < 0.05, P < 0.01). Conclusion During the occurrence of dry eye, there are vitamin A deficiency and retinoic acid abnormal transportion in the body. The acid drug Corni Fructus can improve the status of vitamin A deficiency by increasing the absorption of vitamin A, and then regulate the process of retinol transport. This may be one of the molecular mechanisms of acid entering the liver and improving vision.

R285.5

基于"酸入肝""肝开窍于目"探讨酸味中药及五味间的作用机制（81603312）