目的 研究黔产艾纳香Blumea balsamifera叶中的化学成分并对其进行细胞毒活性评价。方法 使用硅胶柱色谱、薄层色谱、Sephadex LH-20凝胶柱色谱等方法分离纯化艾纳香中化学成分，以宫颈癌（HeLa）细胞株、乳腺腺癌（MCF-7）细胞株、肺腺癌（A549）细胞株、胃癌（MGC-803）细胞株、结肠癌（COLO-205）细胞株为供试细胞株，并采用MTT法对分离纯化的化合物进行细胞毒活性筛选。结果 从艾纳香中分离得到2个倍半萜内酯，通过波谱数据分析鉴定为艾纳香烯N（1）和艾纳香烯F（2）。细胞毒活性筛选结果表明化合物1对供试的5种细胞株均具有明显的抑制活性，其半数抑制浓度（IC₅₀）值为48.730~97.907 μmol/L；化合物2对乳腺腺癌MCF-7细胞株也具有一定的抑制活性，IC₅₀值为91.188 μmol/L。结论 化合物1为新的倍半萜内酯类化合物；活性筛选结果表明2个倍半萜内酯类化合物都具有一定的细胞毒活性。

Objective In order to further study the chemical constituents of Blumea balsamifera and evaluate the cytotoxic activities of the compounds. Methods The chemical components of B. balsamifera were purified by various chromatographic methods. MTT method was employed to screen the cytotoxic activities to the five tumor cells (HeLa, MCF-7, A549, MGC-803 and COLO-205 cells) of the purified compounds. Results Two sesquiterpenoid esters blumeaene N (compound 1) and blumeaene F (compound 2) were isolated from B. balsamifera, of which, blumeaene N was a novel compound. The screening results of cytotoxin activities showed that compound 1 had certain inhibitory activities for the five tumor cells with the IC₅₀ value ranges from 48.730 μmol/L to 97.907 μmol/L; Compound 2 also had a weak inhibitory activity on MCF-7 cell lines with the IC₅₀ value of 91.188 μmol/L. Conclusion Compound 1 is a new sesquiterpenoid ester found from B. balsamifera. Cytotoxic activities screening results indicated that B. balsamifera contains anti-tumor active ingredients.

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国家自然科学基金资助项目（31600280）；国家自然科学基金资助项目（31860074）；贵州省科技计划项目（黔科合支撑[2018]2799号）；黔科合平台人才（2017-5737）；省部共建药用植物功效与利用国家重点实验室开放课题项目（FAMP201704K）