目的 研究丹参水溶性成分促血管生成及心脏保护作用的谱-效关系，揭示其药效物质基础。方法 采用HPLC法建立丹参水溶性成分指纹图谱，以斑马鱼建立不同药效学模型，对丹参水溶性成分促血管生成及心脏保护作用进行评价，利用灰色关联度分析方法，建立丹参水溶性成分促血管生成及心脏保护作用的谱-效关系。结果 在丹参水溶性成分HPLC指纹图谱中，9种水溶性成分与各药效作用均呈正相关，且各共有峰与药效关联度趋势基本一致，其中5号峰（紫草酸）对各药效作用贡献最大。结论 通过谱-效关系研究，阐明了丹参水溶性成分促血管生成及心脏保护作用的物质基础，可为其深入研究奠定基础。

Objective To study the spectrum-effect relationship of angiogenesis activity and protective effect of heart injury of water-soluble components of Salvia miltiorrhiza to reveal the pharmacodynamics material basis. Methods The HPLC fingerprints of water-soluble components of S. miltiorrhiza were established to evaluate the angiogenesis activity and protective effect of heart injury of water-soluble components of S. miltiorrhiza by establishing different models of zebrafish. The spectrum-effect relationship of angiogenesis activity and protective effect of heart injury of water-soluble components of S. miltiorrhiza was established by grey correlation degree analysis. Results Nine water-soluble components in the fingerprints were positively correlated with efficacy of medicine, the association trend of common peaks and efficacy was consistent, and peak 5 (alkannic acid) played the most significant role among nine components. Conclusion Elucidation of the material basis of angiogenesis activity and protective effect of heart injury of water-soluble components of S. miltiorrhiza by researching on the spectrum-effect relationship will provide the basis for the deep research of S. miltiorrhiza.

R285.5

国家重点研发计划项目（2017YFC1701501）；国家重点研发计划项目（2017YFC1702700）；山东省重点研发计划项目（2017CXGC1306）