目的 对青花椒Zanthoxylum schinifolium根皮中抗炎活性成分进行跟踪分离研究。方法 采用硅胶、Sephadex LH-20、MCI以及半制备HPLC等色谱方法进行分离纯化，根据理化性质和现代谱学技术鉴定化合物的结构，以脂多糖（LPS）诱导的RAW264.7细胞炎症模型进行抗炎活性评价。结果 从青花椒根皮的醋酸乙酯部位中共分离得到4个香豆素类化合物，分别鉴定为青花椒素A（1）、7-甲氧基香豆素（2）、异东莨菪内酯（3）和秦皮乙素（4）。在LPS诱导RAW264.7细胞产生一氧化氮（NO）的模型中，化合物1~4均对NO的产生具有抑制作用，半数抑制浓度（IC₅₀）值分别为（0.21±0.03）、（0.92±0.16）、（0.15±0.02）和（0.26±0.04）μmol/L。结论 化合物1为新化合物，是1个罕见的香豆素类化合物，命名为青花椒素A。化合物3为首次从该属植物中分离得到，所有化合物均具有较强的抗NO生成活性。

Objective To study the anti-inflammatory components from the root barks of Zanthoxylum schinifolium. Methods The compounds were isolated and purified by silica gel, MCI, Sephadex LH-20 column chromatographies, and semi-preparative HPLC, and their structures were elucidated by physical and spectroscopic analysis. The anti-inf1ammatory activity was evaluated by the levels of NO in LPS-induced RAW264.7 tested by Griess reagent. Results Four coumarins, namely zantholin A (1), 7-methoxycoumarin (2), isoscopoletin (3), and esculetin (4), were isolated from the root barks of Z. schinifolium. Compounds 1-4 exhibited anti-inflammatory activities on inhibition of lipopolysaccharide (LPS)-induced NO production in RAW 264.7 macrophages with IC₅₀ values of (0.21 ±0.03), (0.92 ±0.16), (0.15 ±0.02), and (0.26 ±0.04) μmol/L, respectively. Conclusion Compound 1 is a new compound, a rare terpenylated coumarin named zantholin A, and compounds 3 is obtained from this plant for the first time. All compounds exhibited anti-inflammatory activities.

R284.1

陕西省重点研发计划（2017SF-365）；陕西省重点研发计划（2017SF-327）；陕西省教育厅自然科学专项项目（16JK1196）