目的 研究昆明山海棠Tripterygium hypoglaucum根中的化学成分。方法 利用硅胶、Sephadex LH-20柱色谱及半制备HPLC进行分离纯化，采用¹H-NMR、¹³C-NMR、ESI-MS等多种波谱技术鉴定获得的化合物结构。结果 从昆明山海棠根乙醇提取物的二氯甲烷部位分离得到6个化合物，分别鉴定为2-（5'，7'-二甲氧基-2'，2'-二甲基-2H-苯骈吡喃-6'-）-3-甲酰基-5，6-二甲氧基-苯骈呋喃（1）、3（R）-（4'，5'-二羟基）-2'，5，7-三甲氧基-6-（3-甲基-2-丁烯基）-异黄酮（2）、3'-香叶草基-5，7，2'，5'-四羟基异黄酮（3）、β-谷甾醇棕榈酸酯（4）、甘油山嵛酸酯（5）、β-谷甾醇（6）。结论 化合物1为新化合物，命名为毛秔子梢素C；化合物2~5为首次从该植物中分离得到。

Objective To study the chemical constitutes from the roots of Tripterygium hypoglaucum. Methods The chemical constituents from 95% EtOH extract of the roots of T. hypoglaucum at room temperature were isolated and prepared by Silica gel column chromatography, Sephadex LH-20 column chromatography, and semi-pre HPLC after dichloromethane extraction, and their structures were elucidated by a variety of spectral and spectroscopic techniques. Results Six compounds were isolated and identified as 2-(5',7'-dimethoxy-2',2'-dimethyl-2H-benzopyran-6'-)-3-formyl-5,6-dimethoxy-benzofuran (1), 3(R)-4',5'-dihydroxy-2',5,7-trimethoxy-6-(3-methyl-but-2-enyl)-isoflavan (2), 3'-geranyl-5,7,2',5'-tetrahydroxyisoflavone (3), β-sitosterolpalmitate (4), docosanoic acid 2',3'-dihydroxypropyl ester (5), and β-sitosterol (6). Conclusion Compound 1 is a new compound, named as hirtellanine C. Compounds 2-5 are isolated from the plants for the first time.

R284.1

国家自然科学基金资助项目（81573577）