目的 建立陈皮饮片HPLC指纹图谱分析方法，为其质量控制提供技术参考。方法 采用HPLC法建立17批陈皮饮片的指纹图谱，通过聚类分析（cluster analysis，CA）、主成分分析（principal component analysis，PCA）、正交偏最小二乘判别分析（orthogonal partial least square discrimina te analysis，OPLS-DA）对指纹图谱进行评价。结果 建立了陈皮饮片指纹图谱，相似度均>0.9，确定了25个共有峰，其中14个峰的变量投影重要性（variable importance projection，VIP）均>1，通过与对照品谱图比对，确定了13号峰为芸香柚皮苷、14号峰为橙皮苷、23号峰为川陈皮素、24号峰为3，5，6，7，8，3'，4'-七甲氧基黄酮、25号峰为桔皮素。结论 该方法简单可靠，可用于陈皮饮片鉴别和质量控制。

Objective To establish an HPLC method for the fingerprints analysis of decoction pieces of tangerine peel, so as to provide reference for the quality control of it. Methods Fingerprints of 17 batches of decoction pieces of tangerine peel were established by HPLC and evaluated by cluster analysis (CA), principal component analysis (PCA) and orthogonal partial least square discriminate analysis (OPLS-DA). Results The method of fingerprint of decoction pieces of tangerine peel was established, the similarities were greater than 0.9. There were 25 common peaks in the HPLC fingerprint, of which variable importance projection (VIP) of 14 peaks were greater than 1. Compared with the spectrogram of reference substances, peak 13 was narirutin, peak 14 was hesperidin, peak 23 was nobiletin, peak 24 was 3,5,6,7,8,3',4'-heptamethoxy flavone, and peak 25 was hesperetin. Conclusion This simple and reliable method can be used for the identification and quality control of decoction pieces of Citri Reticulatae Pericarpium.

R286.2

国家中药标准化项目（ZYBZH-Y-CQ-46）