目的 研究红藤提取物联合5-氟尿嘧啶对人肝癌HepG2细胞生长的抑制作用，并初步探讨其作用机制。方法 体外培养HepG2细胞，以不同质量浓度的红藤提取物作用于细胞，采用MTT法检测其对细胞生长的影响，选择后续实验浓度。将HepG2细胞分为对照组、红藤提取物（40 mg/L）组、5-氟尿嘧啶（10μmol/L）组及联合用药（红藤提取物40 mg/L+5-氟尿嘧啶10 μmol/L）组，采用MTT法检测细胞增殖抑制率，流式细胞仪检测细胞周期，Western blotting法检测细胞中增殖细胞核抗原（PCNA）、细胞周期蛋白D1（Cyclin D1）及细胞周期依赖性蛋白激酶4（CDK4）表达水平。结果 MTT检测结果显示，红藤提取物呈质量浓度依赖性地抑制HepG2细胞增殖，选择其质量浓度为40 mg/L用于后续实验。与对照组比较，红藤提取物组和5-氟尿嘧啶组细胞增殖抑制率显著升高，G₀/G₁期细胞比例显著升高，S期和G₂/M期细胞比例显著降低，细胞中PCNA、Cyclin D1、CDK4蛋白的表达显著降低（P<0.05）。与5-氟尿嘧啶组比较，联合用药能显著抑制HepG2细胞增殖，阻滞细胞周期，抑制细胞中PCNA、Cyclin D1、CDK4蛋白表达（P<0.05）。结论 红藤提取物联合5-氟尿嘧啶能够增强对HepG2细胞生长的抑制作用，其作用机制可能与抑制细胞中PCNA、Cyclin D1、CDK4蛋白表达水平有关。

Objective To investigate the effect of Sargentodoxa cuneata extracts combined with 5-fluorouracil on the growth inhibition of human hepatoma HepG2 cells, and explore its mechanism. Methods HepG2 cells were cultured in vitro and treated with different concentrations of S. cuneata extracts. The effect of S. cuneata extracts on cell growth inhibition was detected by MTT assay, and the subsequent experimental concentration was selected. HepG2 cells were randomly divided into four groups:control group, S. cuneata extracts group (40 mg/L), 5-fluorouracil (10 μmol/L) group, and combination group (S. cuneata extracts 40 mg/L + 5-fluorouracil 10 μmol/L). Cell proliferation inhibition rate was detected by MTT assay and cell cycle was detected by flow cytometry. The expression levels of nuclear antigen PCNA, Cyclin D1, and cell cycle-dependent protein kinase CDK4 were detected by Western blotting. Results The results of MTT assay showed that S. cuneata extracts inhibited the proliferation of HepG2 cells in a concentration-dependent manner (P < 0.05), and S. cuneata extracts with a concentration of 40 mg/L was selected for subsequent experiments. Compared with the control group, the cell proliferation inhibition rate of the S. cuneata extracts group and the 5-fluorouracil group was significantly increased, the proportion of G₀/G₁ phase cells was significantly increased, and the proportion of cells in the S phase and G₂/M phase was significantly decreased (P < 0.05). The protein expression levels of PCNA, Cyclin D1, and CDK4 in the cells were significantly decreased (P < 0.05). Compared with the 5-fluorouracil group, the combination group significantly inhibited the proliferation of HepG2 cells, blocked the cell cycle, and inhibited the protein expression of PCNA, Cyclin D1, and CDK4 in the cells (P < 0.05). Conclusion S. cuneata extracts combined with 5-fluorouracil enhances the growth inhibition of hepatocellular carcinoma HepG2 cells, and its mechanism may be related to the inhibition of the protein expression of PCNA, Cyclin D1, and CDK4 related to the expression levels of PCNA, Cyclin D1, and CDK4 in the inhibited cells.

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重庆市卫生计生委中医药科技项目：红藤联合脉通散外敷治疗恶性肿瘤下肢静脉血栓的临床研究（ZY201702040）