目的 对藏药高杯喉毛花Comastoma traillianum的化学成分进行研究。方法 运用RP-HPLC、柱色谱硅胶、葡聚糖凝胶及RP-C₁₈等多种色谱技术进行分离纯化，并根据理化性质和波谱数据鉴定化合物的结构，并用Griess试剂法测定了化合物对脂多糖（LPS）和γ干扰素（IFN-γ）诱导的小鼠单核巨噬细胞RAW 264.7的抗炎活性，并计算半数抑制浓度（IC₅₀）值评价其抗炎活性。结果 从高杯喉毛花中分离得到了4个裂环环烯醚萜苷类化合物，分别鉴定为高杯喉毛花内脂A（1）、2'-间羟基苯甲酰獐牙菜苷（2）、獐牙菜苦苷（3）、龙胆苦苷（4）。其中化合物1为新化合物，命名为高杯喉毛花内脂A。4个化合物体外抗炎活性测试未显示活性。结论 化合物1~4为首次从高杯喉毛花中分离得到，其中化合物1为新化合物。所有化合物在100 μmol/L的浓度下均未检测到抗炎活性。

Objective To research the chemical constituents in Comastoma traillianum. Methods The chemical constituents from C. traillianum were isolated and purified by column chromatographic methods of silica gel, RP-HPLC, Sephadex LH-20, and RP-C₁₈. Their structures were elucidated by modern spectroscopy methods, including 1D and 2D NMR, HRESIMS, IR, and UV techniques. The anti-inflammatory activity of compounds against LPS and IFN-γ-induced mouse RAW 264.7 macrophages was determined by Griess, and the IC₅₀ value was calculated to evaluate their anti-inflammatory activity. Results Four secoiridoid glycosides (1-4) were isolated from C. traillianum, and identified as comtraide A (1), deacetylcentapicrin (2), swertiamarin (3), and gentiopieroside (4). Compound 1 is a new compound named comtraide A. All compounds were tested for their in vitro anti-inflammatory activity and no activity was found. Conclusion Compounds 1-4 are isolated for the first time from C. traillianum, and compound 1 is a new compound. However, no significant anti-inflammatory activities were detected in all these compounds at concentrations up to 100 μmol/L.

R284.1

国家自然科学基金项目（21562047）；云南民族大学研究生创新基金项目（2018YJCXS275）