目的 研究筒鞘蛇菰Balanophora involucrate全植株的化学成分。方法 利用聚酰胺，正、反相硅胶柱，MCI及半制备型HPLC等进行分离纯化，根据理化性质及波谱数据鉴定化合物的结构，并以脂多糖（LPS）诱导小鼠单核-巨噬细胞（RAW264.7）建立细胞炎症模型，分别采用Griess法和酶联法检测一氧化氮（NO）和白细胞介素-6（IL-6）以评价化合物的抗炎活性。结果 从筒鞘蛇菰全植株75%乙醇提取物分离得到11个木脂素类化合物，分别鉴定为（+）-松脂素（1）、（+）-5'-hydroxypinoresinol（2）、异落叶松脂醇4-O-β-D-吡喃葡萄糖苷（3）、（+）-异落叶松脂素（4）、burselignan（5）、（+）-9-acetoxyisolariciresinol（6）、yunnanensin A（7）、（-）-开环异落叶松脂素-4-O-β-D-吡喃葡萄糖苷（8）、（-）-开环异落叶松脂素（9）、二氢荜澄茄素（10）、secoisolariciresinol-9'-acetate（11）。结论 化合物11为新的天然产物，化合物2、5、7、8、10首次从该属植物中分离得到。所有化合物均表现出较强的抗炎活性。

Objective To study the chemical constituents in the whole herb of Balanophora involucrate. Methods The compounds were isolated and purified using polyamide, silica gel colimu, ODS, MCI gel, and semi-preparative HPLC, and their structures were elucidated by means of physicochemical properties and spectroscopic analysis. The anti-inflammatory activities of all the isolated compounds were evaluated using Griess method and ELISA for the determination of LPS-induced NO and IL-6 releases in inflammation cell model induced by LPS. Results Eleven lignans were isolated from 75% ethyl alcohol extract from the whole herb of B. involucrata and identified as (+)-pinoresinol (1), (+)-5'-hydroxypinoresinol (2), isolariciresinol 4-O-β-D-glucopyranoside (3), (+)-isolariciresinol (4), burselignan (5), (+)-9-acetoxyisolariciresinol (6), yunnanensin A (7), (−)-secoisolariciresinol-4-O-β-D- glucopyranoside (8), (−)-secoisolariciresinol (9), dihydrocubebin (10), and secoisolariciresinol-9'-acetate (11). Conclusion Among them, compound 11 is a new natural product, and compound 2, 5, 7, 8, and 10 are isolated from the genus of Balanophora for the first time. All compounds showed strong anti-inflammatory activities.

国家自然科学基金面上项目（81473423）