目的 采用HPLC对15批不同采集地的蒙药肋柱花药材进行指纹图谱研究，并采用相似度评价蒙药肋柱花药材的质量。方法 采用HPLC进行测定，色谱柱为YMC C₁₈色谱柱（250 mm×4.6 mm，5 μm），流动相为乙腈-0.4%磷酸水-甲醇，梯度洗脱，体积流量0.8 mL/min，检测波长254 nm，柱温30℃。运用色谱软件对指纹图谱进行相似度评价。结果 建立了蒙药肋柱花药材的HPLC指纹图谱，确定了15个共有峰，指认了5个共有峰，15批次肋柱花药材与生成对照指纹图谱的相似度在0.881~0.997。结论 首次建立肋柱花药材的HPLC特征指纹图谱，所建立的指纹图谱测定方法精密度、稳定性和重复性良好。

Objective To study the fingerprints of 15 batches of Mongolian medicinal materials of Lomatogonium rotatum collected from different places by HPLC, and to evaluate the quality of the medicinal materials of L. rotatum by similarity calculation. Methods A total of 15 batches of ribbed flowers were collected by HPLC. Chromatographic column:YMC C18 column (250 mm×4.6 mm, 5 μm), mobile phase:acetonitrile-0.4% phosphoric acid-methanol, gradient elution, flow rate was 0.8 mL/min, detection wavelength was 254 nm, column temperature was 30℃. The similarity of fingerprints was evaluated by using the "Similarity Evaluation System of Chromatographic Fingerprints of Traditional Chinese Medicine 2004A Edition". Results The fingerprints of L. rotatum of Mongolian medicine were established, 15 common peaks were identified, five common peaks were identified, and the similarity among 15 batches of L. rotatum and the fingerprints of control was in the range of 0.881-0.997. The fingerprints of erect column flowers have good precision, stability and reproducibility. Conclusion The characteristic fingerprint of L. rotatum was established for the first time, which not only provides a new scientific basis for the identification and quality control of L. rotatum, but also has important significance for the quality evaluation of L. rotatum.

R284.1

内蒙古政府蒙药标准化重大项目（MB2017-01~03）