目的 研究灰毛康定黄芪Astragalus tatsienensis var.incanus地下部分化学成分。方法 利用RP₁₈、葡聚糖凝胶（Sephadex LH-20）柱色谱以及制备液相色谱等方法进行分离纯化，并通过现代波谱分析及理化性质等手段对所分离得到的化合物进行结构鉴定。结果 从灰毛康定黄芪地下部分甲醇提取物中分离得到4个化合物，分别鉴定为3β-O-α-L-吡喃鼠李糖（1→2）-β-D-吡喃半乳糖（1→2）-β-D-吡喃葡萄糖醛酸-22β-羟基-11-氧代-齐墩果烷-12-烯（1）、金丝桃苷（2）、wighteone（3）、4-羟基苯甲酸（4）。结论 化合物1为新三萜苷类化合物，命名为灰毛康芪甲苷，化合物2~4均为首次从该植物中分离得到。

Objective To study the chemical constituents of the underground parts of Astragalus tatsienensis var. incanus. Methods The compounds were separated and purified by RP₁₈, Sephadex LH-20 column chromatography, as well as preparative liquid chromatography. The structures of the isolated compounds were determined by means of modern spectroscopic analysis and physicochemical properties. Results Four compounds were isolated from the methanol extract of the underground part of A. tatsienensis var. incanus, and identified as 3β-O-α-L-rhamnopyranosyl (1→2)-β-D-galactopyranosyl (1→2)-β-D-glucuronopyranosyl-22β-hydroxy-11-oxo-olean-12-ene (1), hyperoside (2), wighteone (3), and 4-hydroxybenzoic acid (4). Conclusion Compounds 2-4 are isolated from this plant for the first time, and compound 1 is a new triterpene glycosides compound, named astratatincoside A.

R284.1

国家自然科学基金资助项目（31170313）；国家自然科学基金资助项目（81460532）