目的 探讨DNA条形码技术在民族药紫丹参及其近缘物种鉴定中的应用，为紫丹参药材鉴定提供参考。方法 建立紫丹参及其混伪品DNA条形码参照数据库，通过比对序列、分析变异位点、计算遗传距离和构建邻接树等分析数据，并应用该数据库对市场药材进行鉴定。结果 DNA条形码技术可有效鉴别紫丹参及其混伪品。结论 ITS2序列可作为紫丹参及其混伪品的鉴定条形码，该技术在民族药物种基原鉴定、标准制定和市场监管等实际应用中具有重要的应用价值。

Objective To explore the application of DNA barcode technology in identification of ethnic medicine Salvia yunnanensis and its related species, so as to provide reference for identification of S. yunnanensis. Methods A DNA barcode database of S. yunnanensis and its adulterants was successfully constructed. Data were analyzed by aligning sequences, analyzing variation sites, calculating genetic distances, and establishing Neighbor-Joining trees. The database was used to identify 45 S. yunnanensis decoction piece samples purchased from drug stores. Results The results showed that the ITS2 barcodes can be used to identify S. yunnanensis and its adulterants. Conclusion The use of DNA barcoding for market supervision can broaden the application of this technology and provide an excellent inspection method for the supervisory institutions. This study substantially contributes to the protection of public health worldwide.

国家自然科学基金资助项目（31460084）；云南省中青年学术技术带头人后备人才项目（2015HB058）；大理大学-四川滇虹药业合作项目（CDDC2017087）；大理大学博士启动基金资助项目（KYBS201737）