目的 建立HPLC-DAD法同时测定九味肝泰胶囊中尿囊素、人参皂苷Rg₁、人参皂苷Rb₁、三七皂苷R₁、黄芩苷、五味子醇甲、姜黄素、大黄素和大黄酚的方法。方法 采用HPLC法，色谱柱为Ultimate AQ-C₁₈（150 mm×4.6 mm，5.0 μm）；流动相为0.5%磷酸水溶液-（乙腈-甲醇20：80），梯度洗脱，体积流量1.0 mL/min；柱温35℃。结果 尿囊素、人参皂苷Rg₁、人参皂苷Rb₁、三七皂苷R₁、黄芩苷、五味子醇甲、姜黄素、大黄素和大黄酚9种成分能够达到良好分离；其线性范围分别为1.6～160.0μg/mL（r=0.999 7）、1.2～120.0 μg/mL（r=0.999 6）、1.2～120.0 μg/mL（r=0.999 6）、0.4～40.0 μg/mL（r=0.999 2）、4.0～400.0 μg/mL（r=0.999 8）、0.4～40.0 μg/mL（r=0.999 1）、0.16～16.0 μg/mL（r=0.999 1）、0.08～8.00 μg/mL（r=0.999 0）、0.2～20.0 μg/mL（r=0.999 2），平均加样回收率分别为99.3%、100.2%、99.8%、98.3%、99.9%、97.8%、97.8%、102.2%、101.9%，RSD分别为0.6%、0.5%、0.7%、1.1%、0.3%、0.9%、1.4%、1.5%、1.2%。9批次样品中尿囊素、人参皂苷Rg₁、人参皂苷Rb₁、三七皂苷R₁、黄芩苷、五味子醇甲、姜黄素、大黄素和大黄酚质量浓度分别为3.634～3.655、2.523～2.611、2.405～2.424、0.802～0.829、10.362～10.623、0.901～0.921、0.334～0.366、0.142～0.160、0.462～0.479 mg/g。结论 本方法操作简便，测定结果准确可靠，可用于九味肝泰胶囊的质量控制。

Objective To establish HPLC-DAD method for the simultaneous determination of allantoin, ginsenoside Rg₁, ginsenoside Rb₁, notoginsenoside R₁, baicalin, schisandrin, curcumin, emodin and chrysophanol in Jiuwei Gantai Capsules (JGC). Methods The chromatographic separation was achieved on an Ultimate AQ-C₁₈ column (150 mm×4.6 mm, 5.0 μm) with mobile phase consisted of (0.1% phosphate)-(acetonitrile-methanol 20:80) for gradient elution, at the flow rate of 1.0 mL/min; The column temperature was 35℃. Results The linear ranges of allantoin, ginsenoside Rg₁, ginsenoside Rb₁, notoginsenoside R₁, baicalin, schisandrin, curcumin, emodin, and chrysophanol were 1.6-160.0 μg/mL (r=0.999 7), 1.2-120.0 μg/mL (r=0.999 6), 1.2-120.0 μg/mL (r=0.999 6), 0.4-40.0 μg/mL (r=0.999 2), 4.0-400.0 μg/mL (r=0.999 8), 0.4-40.0 μg/mL (r=0.999 1), 0.16-16.0 μg/mL (r=0.999 1), 0.08-8.00 μg/mL (r=0.999 0), and 0.2-20.0 μg/mL (r=0.999 2), respectively. The average recoveries (n=6) were 99.3% (RSD=0.6%), 100.2% (RSD=0.5%), 99.8% (RSD=0.7%), 98.3% (RSD=1.1%), 99.9% (RSD=0.3%), 97.8% (RSD=0.9%), 97.8% (RSD=1.4%), 102.2% (RSD=1.5%), and 101.9% (RSD=1.2%), respectively. The content of the allantoin, ginsenoside Rg₁, ginsenoside Rb₁, notoginsenoside R₁, baicalin, schisandrin, curcumin, emodin, and chrysophanol in nine batches were 3.634-3.655, 2.523-2.611, 2.405-2.424, 0.802-0.829, 10.362-10.623, 0.901-0.921, 0.334-0.366, 0.142-0.160, and 0.462-0.479 mg/g, respectively. Conclusion The method is accurate, sensitive, credible, and repeatable, which can be applied to the quality control of JGC.