目的 建立一种快速鉴别何首乌真伪的方法。方法 通过何首乌及其混伪品的psbA-trnH基因序列，寻找SNP位点并设计特异性引物，对来自于不同产地的何首乌及其3个同属混伪品进行PCR扩增，优化反应体系条件，并对此方法进行考察。结果 建立了何首乌特异性PCR的方法，在退火温度48℃、循环次数30时仅有何首乌能扩增得到191 bp的特异性条带，伪品则无。结论 等位基因特异性PCR鉴别何首乌真伪方法简单、可靠。

Objective To establish a rapid molecular identification method for Fallopia multiflora and its adulterants. Methods Based on psbA-trnH sequences of F. multiflora and its adulterants, the SNP site was searched and the specific primers were designed. The allele-specific PCR amplification of F. multiflora and its adulterants from different producing areas was carried out and the reaction system was optimized. Results When the annealing temperature was raised to 48℃ with 30 cycle number, only the template DNA of F. multiflora could be amplified to obtain the specific 191 bp band whereas the diagnostic PCRs of the other adulterants were all negative. Conclusion It's simple and reliable to identify the authenticity of F. multifloa by allele loci specific PCR.

中央本级重大增减支项目"名贵中药资源可持续利用能力建设"（2060302）