目的 研究内生真菌破除刺五加种子休眠的方法及其内源激素和酶的变化规律。方法 用内生真菌侵染刺五加种子后对种子进行变温层积处理，利用高效液相色谱法测定刺五加种子中内源激素赤霉素（GA₃）、脱落酸（ABA）、吲哚乙酸（IAA）、吲哚丁酸（IBA）及水杨酸（SA）的含量，并且检测体内酶[过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、过氧化物酶（POD）、丙二醛（MDA）]活力变化。结果 5株刺五加内生真菌对种子萌发有明显的促进作用，在变温层积过程中，GA₃、IAA、IBA、SA均有不同程度的上升，ABA呈现明显的下降趋势。POD活力和MDA水平显著下降，CAT和SOD的酶活力显著上升。结论 内生真菌可调控刺五加种子激素和酶的含量，促进种子萌发。

Objective To study the methods of endophytic fungi to break the dormancy of Acanthopanax senticosus seeds and the changes of endogenous hormones and enzymes. Methods The seeds of A. senticosus were stimulated with endophytic fungi, then treated under thermophilic stratification. The content of endogenous hormones such as GA₃, ABA, IAA, IBA, and SA, in the seeds of A. senticosus were tested by HPLC. And the changes of its in vivo enzymes (CAT, SOD, POD, and MDA) activity were tested. Results Five strains of endophytic fungi from A. senticosus apparently promoted the germination of seeds. In the process of fluctuating temperature stratification, the content of GA₃, IAA, IBA, and SA were increased, at the same time, the content of ABA was reduced. The activity of POD and MDA enzymes was significantly reduced, and the enzyme activities of CAT and SOD increased obviously. Conclusion It is suggested that endophytes have significant effect on the content of seed hormones and enzymes, in addition endophytes could promote the initiation of seed germination.

国家"十三五"重点研发计划"东北森林区道地林药资源生态开发利用技术与示范"（2016YFC0500303）；黑龙江省国家科技重大专项和重点研发项目省级资助（GX17C006）；黑龙江省博士后资助项目（LBH-Z17208）