目的 研究白藜芦醇对卵巢癌A2780细胞生长的影响，并探讨其作用机制。方法 运用MTT法检测白藜芦醇（50、100、200、400 μmol/L）对A2780细胞的抑制率；白藜芦醇+pcDNA3.1组（转染pcDNA3.1）、白藜芦醇+pcDNA3.1-SIRT1组（转染pcDNA3.1-SIRT1）均用脂质体法转染，加入白藜芦醇（200 μmol/L）处理；采用实时荧光定量PCR（qRT-PCR）法检测细胞中信息调节因子1（SIRT1）、β-catenin、c-Myc的mRNA水平；Western blotting法检测细胞中SIRT1、β-catenin、c-Myc蛋白表达；流式细胞术检测细胞凋亡率。结果 与对照组比较，白藜芦醇（50、100、200、400 μmol/L）处理组细胞增殖抑制率、凋亡率均显著升高（P<0.05），且白藜芦醇（200 μmol/L）组细胞中SIRT1的mRNA和蛋白水平均显著降低（P<0.05），可失活Wnt信号通路；过表达SIRT1可逆转白藜芦醇对A2780细胞增殖及Wnt信号通路的失活作用。结论 白藜芦醇可调控SIRT1抑制卵巢癌细胞增殖，促进凋亡，其促凋亡机制可能与失活Wnt信号通路有关。

Objective To study the effects of resveratrol on cell proliferation and apoptosis of A2780 cells, and explore its mechanism. Methods MTT assay was used to detect inhibition rate of A2780 cells with resveratrol (50, 100, 200, and 400 μmol/L); Resveratrol + pcDNA3.1 group (transfected pcDNA3.1), and resveratrol + pcDNA3.1-SIRT1 group (transfected pcDNA3.1-SIRT1) were transfected into A2780 cells by liposome method, and treated with resveratrol (200 μmol/L); The mRNA levels of SIRT1, β-catenin, and c-Myc were detected by qRT-PCR. The protein expression of SIRT1, β-catenin, and c-Myc were detected by Western blotting. The apoptosis rate of each group was detected by flow cytometry. Results Compared with the blank control group, the inhibition rate and apoptosis rate were significantly increased (P <0.05) in A2780 cells with resveratrol (50, 100, 200, and 400 μmol/L), and the mRNA and protein levels of SIRT1 were significantly decreased (P <0.05) in the resveratrol (200 μmol/L) group, which could inactivate the Wnt signaling pathway. Overexpression SIRT1 reversed the inhibitory effect of resveratrol on A2780 cell proliferation and the inactivation of Wnt signaling pathway. Conclusion Resveratrol can inhibit ovarian cancer cell proliferation and promote apoptosis in ovarian cancer cells by regulating SIRT1, and its pro-apoptotic mechanism may be relate to inactivated Wnt signaling pathway, which will provide a basis for resveratrol treatment of ovarian cancer.