目的 研究补益脾胃元气类方药人参、大补元煎、洗心汤对β淀粉样蛋白诱导的海马神经干细胞（neural stem/progenitor cells，NSPCs）活力与迁移能力的影响，探讨其对海马神经元修复和再生的作用。方法 从孕14~16 d Wistar大鼠胎鼠分离培养NSPCs并鉴定，并随机分为空白组、Aβ_1-42处理组、正常脑脊液对照组、安理申组及补益脾胃元气方药人参、大补元煎、洗心汤保护组，共7组。以25 μmol/L Aβ_1-42干预NSPCs，与抽取的终体积分数为20%的不同方药含药脑脊液作用第3代神经干细胞48 h。采用CCK-8实验检测各组NSPCs的活力，Transwell实验和划痕实验检测细胞迁移，流式法检测各组细胞内活性氧（ROS）含量。结果 免疫荧光染色鉴定NSPCs特异性蛋白SOX2染色为阳性；CCK-8检测结果表明，补益脾胃元气方药各组海马NSPCs活性显著增高（P<0.01）；Transwell实验和划痕实验检测结果显示，补益脾胃元气方药各组NSPCs迁移能力显著增强（P<0.05、0.01）；流式法检测ROS含量结果表明，补益脾胃元气方药各组NSPCs内ROS水平显著降低（P<0.01）。结论 补益脾胃元气方药含药脑脊液对大鼠海马NSPCs活力与迁移能力具有促进作用。还可拮抗Aβ诱导的氧化应激，同时其有效成分易于透过血脑屏障，可能发挥更强的神经保护作用。

Objective To study the effects of prescriptions including Ginseng, Dabuyuan Decoction, and Xixin Decoction for tonifying spleen and stomach on the activity and migration ability of neural stem/progenitor cells (NSPCs) induced by β-amyloid protein (β-amyloid), and investigate the effect of hippocampal neuronal repair and regeneration. Methods NSPCs were isolated from fetal rats of 14-16 d gestation and identified. It was randomly divided into seven groups:blank group, blank group treated with Aβ_1-42, control group of normal cerebrospinal fluid, control group of Aricept, prescription of Tonifying Spleen and Stomach and Yuanqi (Ginseng, Dabu Yuan Decoction, and Xixin Decoction) Protection groups. The third generation of neural stem cells were treated with 25 μmol/L Aβ_1-42 for 48 h. The activity of NSPCs in each group was detected by transwell assay and CCK-8 assay and the migration was detected by scratch test. The content of reactive oxygen species (Ros) in the cells was detected by flow cytometry. Results By immunofluorescence staining, NSPCs specific protein Sox2 staining was positive. The CCK-8 detection results showed that the activity of NSPCs in hippocampus was significantly increased in the groups of Tonifying Spleen and Stomach Yuanqi Prescription (P < 0.01); The results of Transwell experiment and scratch test showed that the migration ability of NSPCs was significantly increased (P < 0.05, 0.01). The flow cytometry showed that the content of ROS in the NSPCs of spleen and stomach prescription groups decreased significantly (P < 0.01). Conclusion The prescriptions for invigorating spleen and stomach promoted the activity and migration of NSPCs in rat hippocampal neural stem cells. The oxidative stress induced by Aβ can be antagonized and its active ingredients can easily penetrate the blood-brain barrier to exert a stronger neuroprotective effect.

国家自然科学基金资助项目（81674042）；陕西省自然科学基础研究计划-重大基础研究项目（2017ZDJC-15）