目的 研究皂角刺Gleditsiae Spina醋酸乙酯部位的化学成分。方法 采用硅胶、Toyopearl HW-40C、Sephadex LH-20、MCI Gel CHP-20和ODS柱色谱法及半制备型高效液相色谱进行分离纯化，根据其理化性质和波谱数据鉴定化合物结构。结果 从皂角刺醋酸乙酯部位分离得到18个化合物，分别鉴定为莨菪亭（1）、（-）-（7R，8S）-赤式-愈创木基丙三醇（2）、5-甲氧基-愈创木基丙三醇（3）、（-）-（7R，8R）-苏式-愈创木基丙三醇（4）、3，4'-dihydroxypropiophenone（5）、二氢阿魏酸（6）、对羟基苯甲酸（7）、indole-3-carbaldehyde（8）、原儿茶酸甲酯（9）、原儿茶醛（10）、丁香酸（11）、2-guaiacylpropane-1，3-diol（12）、（2R^*，3R^*，4S^*）-2，3-二脲基-4-羟基四氢呋喃（13）、3-（2-羰基-丙基）-3-羟基-吲哚啉-2-酮（14）、核桃素D（15）、C-藜芦酰乙二醇（16）、3-（4-hydroxyl-3-methoxyphenyl）-propan-1，2-diol（17）、（-）-丁香脂素（18）。结论 18个化合物均为首次从该植物中分离得到。

Objective To investigate the chemical constituents from the ethyl acetate extract of Gleditsiae Spina. Methods The chemical constituents were isolated and identified by chromatography on silica gel, Toyopearl HW-40C, Sephadex LH-20, MCI Gel CHP-20, ODS, and RP-HPLC. Their structures were elucidated on the basis of physicochemical properties and spectral analyses. Results Eighteen compounds were isolated from the ethyl acetate extract of Gleditsiae Spina, and identified as scopoletin (1), (-)-(7R,8S)-erythro-guaiacylglycerol (2), 5-methoxy-guaiacylglycerol (3), (-)-(7R,8R)-threo-guaiacylglycerol (4), 3,4'-dihydroxypropiophenone (5), dihydroferulic acid (6), p-hydroxybenzoic acid (7), indole-3-carbaldehyde (8), protocatechuic acid methyl (9), protocatechuic aldehyde (10), syringic acid (11), 2-guaiacylpropane-1,3-diol (12), (2R^*,3R^*,4S^*)-2,3-diguaiacyl-4-hydroxyl tetrahydrofuran (13), 3-(2-oxopropyl)-3-hydroxy-indolin-2-one (14), juglanin D (15), C-veratroylglycol (16), 3-(4-hydroxyl-3-methoxyphenyl)-propan-1,2-diol (17), and (-)-syringaresinol (18). Conclusion Eighteen compounds are isolated from this plant for the first time.

中央引导地方科技重大发展专项：河南道地大宗药材种质评价及集约化种植与示范（14104349）