目的 研究鸢尾属2种植物膜苞鸢尾Iris scariosa和中亚鸢尾Iris bloudowii中的抗炎活性成分。方法 利用正、反相硅胶柱色谱、凝胶柱色谱、反相薄层制备色谱、半制备液相色谱等技术进行分离纯化，根据波谱学数据（MS、¹H-NMR、¹³C-NMR）进行化合物的结构鉴定。用脂多糖（LPS）诱导小鼠巨噬细胞RAW264.7炎症模型对分离到的化合物进行抗炎活性筛选。结果 从膜苞鸢尾的根及根状茎中分离得到5个化合物，分别鉴定为鸢尾苷（1）、野鸢尾苷（2）、尼鸢尾苷（3）、芒果新苷（4）、芒果苷（5）。从中亚鸢尾的根及根状茎中分离得到了7个化合物，分别鉴定为尼鸢尾苷（3）、尼鸢尾黄素（6）、4'-羟基-5，3'-二甲氧基-6，7-亚甲二氧基异黄酮（7）、5，5'-二羟基-3'，4'-二甲氧基-6，7-亚甲二氧基异黄酮（8）、4'-羟基-5，3'-二甲氧基-6，7-亚甲二氧基异黄酮-4'-O-β-D-吡喃葡萄糖苷（9）、irifloside（10）和尼鸢尾黄素-4'-[O-β-D-吡喃葡萄糖基-（1"'→6"）-β-D-吡喃葡萄糖苷]（11）。化合物2、3、5、6、7、9、11及此前报道的从膜苞鸢尾中分离得到的野鸢尾黄素和irilone均对LPS诱导的RAW264.7细胞NO分泌有一定的抑制作用。结论 化合物1~5是首次从膜苞鸢尾中分离得到，化合物4、6~11是首次从中亚鸢尾中分离得到。生物活性结果提示化合物2、3、5、6、7、9、11以及野鸢尾黄素和irilone具有潜在的抗炎活性。

Objective To study the anti-inflammatory constituents from the roots and rhizomes of Iris scariosa and I. bloudowii. Methods The chemical constituents were isolated and purified by column chromatography (silica gel, ODS, and sephadex LH-20), RP-preparative TLC, and semi-preparative HPLC. Their structures were identified by spectroscopic methods (MS, ¹H-NMR, and ¹³C-NMR). The anti-inflammatory activities of the compounds were evaluated by the inhibition against NO production in lipopolysaeeharide (LPS) activated RAW 264.7 macrophages. Results Five compounds including tectoridin (1), iridin (2), nigricin 4'-O-β-D-glucoside (3), neomangiferin (4), and mangiferin (5) were isolated and identified from the roots and rhizomes of I. scariosa. Besides, seven compounds, nigricin4'-O-β-D-glucoside (3), nigricin (6), nigricanin (7), 5,5'-dihydroxy-3',4'-dimethoxy-6,7-methylenedioxyisoflavone (8), nigricanin-4'-O-β-D-glucopyranoside (9), irifloside (10), and nigricin-4'-[O-β-D-glucopyranosyl-(1"'→6")-β-D-glucopyranoside] (11) were isolated and identified from the roots and rhizomes of I. bloudowii. Compounds 2, 3, 5, 6, 7, 9, 11, and previously reported chemical constituents of I. scariosa irigenin and irilone could inhibit NO production in RAW 264.7 macrophages to some extent. Conclusion Compounds 1 -5 are isolated from I. scariosa and compounds 4 and 6 -11 are isolated from I. bloudowii for the first time, respectively. The results of bioactivity assays indicated that compounds 2, 3, 5, 6, 7, 9, 11, and other two compounds irigenin and irilone isolated and purified from I. scariosa earlier are potential anti-inflammation agents.

"十二五"重大新药创制科技重大专项（2013ZX09103001-019-001）；西安医学院青年科研基金（2016QN19）；西安医学院大学生创新创业训练项目（2015DXS1-21）