目的 研究银柴胡Stellaria dichotoma的生物碱成分及其抗炎活性。方法 利用硅胶柱色谱、MCI柱色谱和反相制备液相色谱等手段进行分离纯化，并通过¹H-NMR、¹³C-NMR等波谱技术进行结构鉴定。采用脂多糖（LPS）诱导的小鼠巨噬细胞RAW264.7炎症反应模型，探究银柴胡中卡巴林生物碱类对巨噬细胞经LPS刺激后产生的炎症因子[肿瘤坏死因子-α（TNF-α）及白细胞介素-6（IL-6）]的影响，评价其抗炎活性。结果 从银柴胡正丁醇萃取物中分离得到9个卡巴林生物碱，分别鉴定为geleboline A（1）、geleboline B（2）、cordysinin E（3）、3，10-dihydroxy-β-carboline（4）、6-hydroxy-3-methoxycarbonyl-β-carboline（5）、1-methyl-3-（2-hydroxypropan-2-yl）-2-（5-methoxy-9H-β-carbolin-1-yl）-cyclopentanol（6）、2-aldehydetetra-hydroharmine（7）、pegaharmine J（8）、pegaharmine K（9）。抗炎活性检测表明，化合物1～9对LPS刺激的巨噬细胞IL-6炎症因子均具有较强的抑制作用。结论 所有化合物均为首次从该植物中分离得到，其中化合物1、2对炎症因子IL-6的抑制作用最强，其IC₅₀值最小仅为5.21 μg/mL，该研究首次发现银柴胡的抗炎成分。

Objective To study the alkaloids and its anti-inflammatory effect of Stellaria dichotoma. Methods The chemical constituents of S. dichotoma were isolated by different column chromatographic techniques, including silica gel, MCI, and reversed phase prep-HPLC. The structures of these compounds were identified by a comprehensive analysis of the spectroscopic data. LPS-induced RAW264.7 inflammatory cell model was established to evaluate the effect of carboline alkaloids in S. dichotoma on proinflammatory factors (TNF-α and IL-6) of RAW264.7 inflammatory cells. Results Nine compounds were isolated from Stellaria dichotoma. The compounds were identified as geleboline A (1), geleboline B (2), cordysinin E (3), 3,10-dihydroxy-β-carboline (4), 6-hydroxy-3-methoxycarbonyl-β-carboline (5), 1-methyl-3-(2-hydroxypropan-2-yl)-2-(5-methoxy-9H-β-carbolin-1-yl)-cyclopentanol (6), 2-aldehydetetra-hydroharmine (7), pegaharmine J (8), pegaharmine K (9). The anti-inflammatory activity assay showed that these nine compounds all had strong inhibited effect on production of IL-6 in RAW264.7 inflammatory cell model. Conclusion All the compounds are isolated from this plant for the first time. Activity assay showed that compound 1 and 2 presented better inhibition for proinflammatory factor IL-6, the minimum IC₅₀ is 5.21 μg/mL, and it is the first time to discover the anti-inflammatory constituents of S. dichotoma.