目的 对云南香料烟的化学成分进行研究，试图从中发现新活性化合物。方法 运用硅胶、凝胶及RP-HPLC等多种色谱技术进行分离纯化，并根据理化性质和波谱数据鉴定化合物的结构。结果 从云南香料烟中分离得到2个烷基取代的黄酮类化合物，分别鉴定为2'-羟基-7-（2-羟乙基）-8-甲氧基-黄酮（1）和烟草黄酮A（2）。对化合物1和2进行了抗菌活性筛选，其对耐甲氧西林金黄色葡萄球菌（MRSA）菌株的MIC₉₀值分别为（38±4）和（33±3）μg/mL。结论 化合物1为新化合物，命名为香料烟黄酮A。化合物2首次从香料烟中分离到，化合物1和2均具有显著的抗菌活性。

Objective To find the new biological compounds, the research of the chemical constituents in the leaves of Nicotiana tabacum had been carried out. Methods The chemical constituents in the leaves of N.tabacum were isolated by silica gel, Sephadex LH-20 column chromatographies, and RP-HPLC methods. The structures of isolations were detected by using various spectroscopic techniques, including extensive 1D and 2D NMR techniques. Results Two C-alkylated flavones were isolated from 95% ethanol extract of plant, which identified as 2'-hydroxy-7-(3-hydroxypropyl)-8-methoxy-flavone (1) and tabaflavone A (2). Compounds 1 and 2 were evaluated for their anti-methicillin-resistant Staphylococcus aureus (anti-MRSA) activity. Conclusion Compound 2 is isolated from the leaves of N.tabacum for the first time. Compound 1 is a new compound named orientalflavone A. Compounds 1 and 2 also show good anti-MRSA activity with MIC₉₀ value of (38 ±4) and (33 ±3) μg/mL, respectively.

国家自然科学基金资助项目（21462051）；中国烟草总公司科技项目（110201502006）；云南中烟科技项目（2016JC03）