目的 建立一测多评法同步测定茯苓中4种三萜类成分的含量，并验证该方法的准确性和可行性。方法 以茯苓酸为内参物，建立其与去氢茯苓酸、去氢土莫酸和松苓新酸的相对校正因子（f）与相对保留时间，计算3种成分的含量，实现一测多评，并将一测多评法测得的结果与外标法比较，验证一测多评的可行性。结果 17批不同产地茯苓样品中4个活性成分，采用f的计算值与外标法的实测值之间无显著性差异。结论 在对照品缺乏的情况下，以茯苓酸为内参物同时测定去氢茯苓酸、去氢土莫酸、松苓新酸的含量是可行的，一测多评法可用于茯苓中三萜类成分的定量评价研究。

Objective To establish a new method and validate its feasibilities by the simultaneous quantitative assay of four triterpenes in Poria cocos. Methods A new quality evaluation method of multi-components by single marker (QAMS) was established and validated for P. cocos. Pachymic acid (PA), dehydropachymic acid (DPA), dehydrotumulosic acid (DTA), and dehydrotrametenolic acid (DMA) were selected as analytes while pachymic acid was chosen as internal reference substance to evaluate the quality. The relative correction factor (RCF) of pachymic acid to the other three triterpenes were calculated. The method was evaluated by the comparison of quantity between external standard method and QAMS method. Results The contents of four triterpenes in 17 batches of P. cocos from QAMS method were not significantly different from those from external standard method. Conclusion The method with a single marker is accurate and feasible to determine PA, DPA, DTA, and DMA when some authentic standard substance are unavailable.

国家中医药管理局项目（ZYBZH-Y-AH-01）；国家重点研发计划项目（2017YFC1701605）；安徽省科技厅公益联动项目（1501Id04004）；安徽道地中药材品质提升协同创新中心项目（皖教科[2013]2号）；安徽高校科研创新团队项目（2016hz23），大学生创新创业项目（2017149）