目的 建立玄参药材中哈巴苷、类叶升麻苷、安格洛苷C、哈巴俄苷、肉桂酸5种成分的一测多评法，验证该法在玄参质量评价中的准确性和可行性。方法 以哈巴苷为内参物，分别建立肉桂酸、类叶升麻苷、安格洛苷C、哈巴俄苷的相对校正因子（f），并计算其含量，实现一测多评。与外标法所得结果进行比较，对一测多评价法的可行性进行验证。结果 建立的相对校正因子分别为f_{哈巴苷/肉桂酸}=0.060（RSD=0.81%）、f_{哈巴苷/类叶升麻苷}=0.068（RSD=0.53%）、f_{哈巴苷/安格洛苷C}=0.197（RSD=1.82%）、f_{哈巴苷/哈巴俄苷}=0.142（RSD=1.17%），差异性较小；25批玄参中肉桂酸、类叶升麻苷、安格洛苷C、哈巴俄苷含量的实测值与计算值无显著差异。结论 一测多评法控制玄参药材的质量是准确、可行的。

Objective To establish a measurement method for the content of five compounds (acteoside, harpagide, harpagoside, angoroside-C, and cinnamic acid) in Scrophularia Radix quantitative analysis multi-components by single-marker (QAMS), and verify the accuracy and feasibility of QAMS in the quality control. Methods Taking harpagide as internal standard substance, the relative correlation factor (RCF) of acteoside, cinnamic acid, harpagoside, and angoroside C was established. And the content of each component in Scrophularia Radix was determined by the above-mentioned RCF. In order to prove the scientificity and feasibility of this method, the results were compared with the external standard method. Results The relative correction factors of acteoside, cinnamic acid, harpagoside, and angoroside-C were 0.068 (RSD=0.53%), 0.060 (RSD=0.81%), 0.142 (RSD=1.17%), 0.197 (RSD=1.82%). No significant differences were found among the quantitative results of four components in 25 batches of Scrophularia Radix determined by the two methods. Conclusion It is feasible and accurate to evaluate the quality of Scrophularia Radix by QAMS.

国家中药标准化项目（ZYBZH-Y-CQ-46）