目的 研究菊科泽兰属植物华泽兰Eupatorium chinense根部的化学成分。方法 采用正相硅胶柱色谱、制备薄层色谱及制备型HPLC等方法分离纯化，利用NMR、MS等波谱学方法鉴定化合物结构。结果 从华泽兰醋酸乙酯部位分离鉴定了19个化合物，鉴定为泽兰素（1）、1-[2-（1-acetoxymethyl-vinyl）-6-hydroxy-benzofuran-5-yl]-ethanone（2）、6-hydroxy-3β-methoxytrematone（3）、euparone（4）、8-methoxy-9-hydroxythymol（5）、dehydroespeleton（6）、8-methoxy-9-hydroxythymol 3-O-angelate（7）、9-hydroxythymol（8）、4-hydroxycinnamic acid methyl ester（9）、对羟基苯甲醛（10）、8，9-dehydro-10-hydroxythymol（11）、邻苯二甲酸二异丁基酯（12）、邻苯二甲酸二正丁酯（13）、对香豆酸（14）、dihydrocoumarin（15）、methylcaffeate（16）、2，5-dimethylphenol（17）、1H-吲唑（18）、（Z）-3-（hydroxymethyl）-7-methylocta-2，6-dienly1 acetate（19）。结论 首次对华泽兰根部的化学成分进行研究，其中化合物2、3、5～11为首次从华泽兰中分离得到，化合物6、9、12、14～19为首次从泽兰属中分离得到，化合物1～4为苯并呋喃类化合物，是泽兰属中的特征性成分。

Objective To study chemical constituents of the roots of Eupatorium chinense. Methods The chemical constituents were separated and purified by the normal phase silica gel column chromatography, preparative thin-layer chromatography, and preparative HPLC. Their structures were determined by various spectral data. Results Nineteen compounds were isolated from the acetic ether extract of E. chinense and the structures were identified as euparin (1), 1-[2-(1-acetoxymethyl-vinyl)-6-hydroxy-benzofuran-5-yl]-ethanone (2), 6-hydroxy-3β-methoxytrematone (3), euparone (4), 8-methoxy-9-hydroxythymol (5), dehydroespeleton (6), 8-methoxy-9-hydroxythymol 3-O-angelate (7), 9-hydroxythymol (8), 4-hydroxy cinnamic acid methyl ester (9), p-hydroxy benzaldehyde (10), 8,9-dehydro-10-hydroxythymol (11), diisobutyl phthalate (12), dibutyl phthalate (13), p-coumaric acid (14), dihydrocoumarin (15), methylcaffeate (16), 2,5-dimethylphenol (17), 1H-indazole (18), and (Z)-3-(hydroxymethyl)-7-methylocta-2,6-dienly1 acetate (19). Conclusion The chemical constituents are investigated and identified from the roots of E. chinense for the first time. Among them, compounds 2, 3, 5-11 are isolated from E. chinense for the first time, and compounds 6, 9, 12, 14-19 are isolated from the genus of Eupatorium for the first time. Compounds 1-4 are benzofurans, which are the characteristic constituents in Eupatorium genus.

国家自然科学基金青年科学基金项目（81803383）；湖北省教育厅科学技术研究项目（Q20181201）