目的 研究西藏黑木耳Auricularia auricula中1种新的凝集素。方法 采用凝胶柱色谱和离子交换色谱进行分离纯化，运用基质辅助激光解吸-电离飞行时间质谱（MALDI-TOF/TOF）测定相对分子质量，通过N-末端序列检测与UniProt数据库比对得到氨基酸序列。结果 从西藏黑木耳分离得到1种相对分子质量为18 913.22的凝集素，其N-末端序列为ITAPTTTSSAATE。含有4条肽段，其氨基酸序列分别为QIDAERK、TNHSVVTWNDK、RLNFTAGNPFPR、VRELEQQVDSMTK。结论 在已有的黑木耳蛋白质质谱库中未发现该序列的存在，推断其为黑木耳中的新型蛋白质，并确定是1种新的凝集素。

Objective To extract and isolate the lectin from Tibetan medicine Auricularia auricula. Methods The lectin from Tibet A. auricula was separated by sephadex column chromatography and ion exchange chromatography, and then its relative molecular weight was determinated by using matrix assisted laser desorption ionization time of flight mass spectrometry (5 800 MALDI-TOF/TOF). N-terminal sequential detection results suggested the amino acid sequences by comparing with the UniProt database. Results A kind of lectin was obtained from the separation of Tibet A. auricula with the molecular weight of of 18 913.22, in which the N-terminal amino acid sequence was detected as ITAPTTTSSAATE by the full automatic protein polypeptide sequencing instrument. The amino acid sequences of four peptide fragments were QIDAERK, TNHSVVTWNDK, RLNFTAGNPFPR, and VRELEQQVDSMTK. Conclusion These sequences are not found in the existing protein database of A. auricula, indicating that the isolated lectin should be a new type protein and it is confirmed as a new kind of lectin.

西藏自治区2016厅校联合基金项目（2016ZR-NQ-01）；西藏自治区2016厅校联合基金（2016ZR-NZ-03）