目的 基于整合药理学思路和方法，对经方当归贝母苦参丸治疗前列腺疾病的物质基础及分子机制进行初步分析。方法 利用中药整合药理学计算平台（TCM-IP，www.tcmip.cn）预测当归贝母苦参丸治疗前列腺疾病的主要活性成分及作用靶点，筛选关键靶标并进行通路富集分析，构建当归贝母苦参丸“中药材-核心成分-关键靶标-主要通路”多维网络，探索其治疗前列腺疾病的可能机制。结果 筛选出当归贝母苦参丸治疗前列腺疾病的候选关键靶标532个，获得候选关键靶标的gene ontology（GO）分析结果1 840条，KEGG通路富集分析结果194条。“中药材-核心成分-关键靶标-主要通路”网络关联分析结果获得当归贝母苦参丸治疗前列腺疾病的核心成分65个（当归29个、浙贝母11个、苦参26个，其中有1个成分为当归和浙贝母的共有成分），作用于转录因子结合、凋亡负性调控等关键靶标，通过雌激素、凋亡、趋化因子等信号通路，发挥细胞周期、细胞凋亡与增殖失衡调控等作用，这可能是其治疗前列腺疾病的相关分子机制。结论 当归贝母苦参丸是以多成分交互作用于多靶点，通过多通路参与调控良性前列腺增生症（BPH）、前列腺癌等疾病发生发展过程，从而发挥一定的治疗作用。

Objective To analyze the material basis and molecular mechanisms of Danggui Beimu Kushen (DBK) Pills in treating prostatic diseases based on the method of integrated pharmacology. Method The platform of Integrative Pharmacology of Traditional Chinese Medicine (TCM-IP, www.tcmip.cn) was utilized to predict the main active ingredients and functional targets of DBK Pills in treating prostatic disease, key targets were screened for enrichment analysis of pathways, and the network of "herb-core component-key target-main pathway" was constructed, and the possible mechanisms of DBK Pills in treating prostatic diseases were explored. Results A total of 532 candidate key targets for the treatment of prostatic diseases by DBK Pills were predicted, and 1 840 terms of gene function and 194 signal pathways were analyzed by gene ontology (GO) and KEGG, respectively. The network analysis of "herb-core component-key target-main pathway" showed that 65 core components were predicted, including 29 ingredients from Angelica sinensis, 11 from Fritillaria thunbergii and 26 from Sophora flavescens. Those predicted components acted on the key targets of prostatic diseases, such as transcription factor binding, negative regulation of apoptosis, et al, through the estrogen, apoptosis, chemokines and other signal pathways, and thus played a role in the regulation of cell cycle, apoptosis and proliferation imbalance, which might be the molecular mechanisms of DBK Pills for the treatment of prostatic disease.Conclusion DBK Pills regulate the development of BPH, prostate cancer and other diseases through multiple pathways with multi-component interacting with multiple targets.

山东省重点产业关键技术项目（2016CYJS08A01-4，2016CYJS08A01-3）；山东省重点研发计划（2016ZDJS07A12）