目的 建立酸枣仁饮片汤剂质量评价方法。方法 收集10批酸枣仁饮片，制备成饮片汤剂。以出膏率和指标成分含量为指标，优化提取工艺。采用HPLC-UV/ELSD技术，建立多维度酸枣仁饮片汤剂指纹图谱。以相似度及相对保留时间、相对峰面积RSD综合评价10批酸枣仁饮片汤剂的质量。建立LC-MS/MS同时测定乌药碱、木兰花碱、维采宁、斯皮诺素、当药黄素、山柰酚-3-O-芸香糖苷、6'"-阿魏酰斯皮诺素、酸枣仁皂苷A和B 9种指标成分含量的方法，并应用于酸枣仁饮片汤剂的质量评价。结果 10批酸枣仁饮片汤剂的平均出膏率为20.77%。在HPLC-UV 227、335 nm和HPLC-ELSD指纹图谱中分别标定了3、4、2个共有峰，不同检测波长下指纹图谱的相似度为0.942～0.988；相对保留时间和相对峰面积RSD均小于3%。9种指标成分在相应的浓度范围内线性关系良好，相关系数r均>0.999 0；平均加样回收率为87.40%～110.42%，RSD均<4.12%。10批酸枣仁饮片汤剂中9种指标性成分的质量分数分别为乌药碱0.25～0.27 mg/g、木兰花碱3.2～3.6 mg/g、维采宁0.077～0.084 mg/g、斯皮诺素0.82～0.89 mg/g、当药黄素0.007 6～0.008 5 mg/g、山柰酚-3-O-芸香糖苷0.057～0.060 mg/g、6'"-阿魏酰斯皮诺素0.32～0.35 mg/g、酸枣仁皂苷A 0.83～0.91 mg/g、酸枣仁皂苷B 0.095～0.110 mg/g。结论 建立了稳定、可靠的酸枣仁饮片汤剂质量评价方法，为后续药动学和药效学实验提供稳定的物质基础，同时为含酸枣仁的成方制剂质量控制提供依据。

Objective To establish a quality evaluation system for slice decoction of Ziziphi Spinosae Semen (SZR). Methods Ten batches of SZR slice were collected and prepared into decoction, and then the decoctions of SZR were prepared as freeze-dried powders. The extraction rate and the content of the index components were used as indicators to optimize the extraction process. Multi-dimensional chemical fingerprints were established by HPLC coupled with UV detector and ELSD detector. The similarity, RSD of relative retention time, and RSD of relative peak area were analyzed to comprehensively evaluate the quality of ten batches of SZR slice decoction. A sensitive method based on ultra-HPLC coupled with triple quadrupole mass spectrometry (UPLC-MS/MS) was established and validated for quantitative analysis of the nine compounds in SZR slice decoction, including coclaurine, magnoflorine, vicenin Ⅱ, spinosin, swertisin, kaempferol-3-O-rutinoside, 6'"-feruloylspinosin, jujuboside A, and jujuboside B. Consequently, the developed methods were successfully applied to the quality assessment of SZR slice decoction. Results The average dry extraction rate of ten batches of SZR slice decoction was 20.77%. Three, four, and two common peaks were identified in the HPLC-UV 227 nm, 335 nm, and HPLC-ELSD fingerprints. The similarity of the fingerprints at different detection wavelengths was in the range of 0.942-0.988, relative retention time and relative peak area RSD% were less than 3%. The method validation results indicated that the methods were simple, specific and reliable. All the compounds showed good linearity (r > 0.999 0) with a relatively wide concentration range, acceptable recovery at 87.40%-110.42%, and RSD% less than 4.12%. The content of nine chemical compounds in ten batches of SZR slice decoction were as follows:0.25-0.27 mg/g of coclaurine, 3.2-3.6 mg/g of magnoflorine, 0.077-0.084 mg/g of vicenin Ⅱ, 0.82-0.89 mg/g of spinosin, 0.007 6-0.008 5 mg/g of swertisin, 0.057-0.060 mg/g of kaempferol-3-O-rutinoside, 0.32-0.35 mg/g of 6'"-feruloylspinosin, 0.83-0.91 mg/g of jujuboside A, and 0.095-0.110 mg/g of jujuboside B. Conclusion A stable and reliable quality evaluation method of SZR slice decoction was established, which provides a stable material basis for the follow-up pharmacokinetic and pharmacodynamic experiments and provides a reference for the quality control of the prescription containing SZR at the same time.

国家自然科学基金青年基金资助项目（81603289，81603251）；山西省研究生联合培养基地人才培养项目（2017JD01）；山西省科技攻关计划-振东专项（2016ZD0105）；山西省基础研究计划项目自然科学基金项目（2015011105）；地产中药功效物质研究与利用山西省重点实验室（201605D111004）；山西大学引进人才事业发展经费（226545022，226545012，226545006）