目的 研究产自丽江的玛咖Lepidium meyenii的干燥根茎的化学成分和生物活性。方法 样品经90%乙醇水提取后，再经硅胶柱、微孔树脂（MCI）和高效液相色谱法等方法分离有效成分，通过核磁共振技术（包括二维核磁HSQC、HMBC和COSY）进行结构鉴定，采用MTT法对新化合物进行抗肿瘤活性测试。结果 从玛咖根中分离得到3个玛咖酰胺类化合物，分别鉴定为N-（3，4-二甲氧基苄基）十六碳-9Z-烯酰胺（1）、N-苄基-13-酮基-9E，11E-十八碳二烯酰胺（2）和N-（3，4-二甲氧基苄基）-十六碳酰胺（3）。结论 化合物1为新化合物，命名为玛咖新胺A；该化合物对人5种癌细胞（NB4、A549、SHSY5Y、PC3、MCF7）没有明显细胞毒活性。

Objective To study the chemical constituents of the dried roots of Lepidium meyenii cultivated in Lijiang. Methods The samples were extracted by 90% alcohol, and then isolated by silica column, MCI, and HPLC. The structures of the isolated compounds were elucidated by NMR techniques including 2D NMR such as HSQC, HMBC, and COSY. The cytotoxic activity of the new compound against five cell lines (NB4, A549, PC3, SHSY5Y, and MCF7) was evaluated by MTT methods. Results Three macamides were isolated and identified as N-(3,4-dimethoxybenzyl) hexadec-9Z-enamide (1), N-benzyl-13-oxo-9E,11E-octadecadienamide (2) and N-(3,4-dimethoxybenzyl)-hexadecanamide (3) from this plant. Conclusion Compound 1 is a new compound named macalepidiumide A and shows no significant cytotoxic activity.

国家自然科学基金资助项目（21262048，31560099）；烟草技术与装备研究重点实验室开放项目（2016GYSYS03）