目的 构建博落回Macleaya cordata果化学成分指纹图谱，建立博落回果品质评价方法。方法 根据湖南省长沙市地方标准2009年版测定博落回中原阿片碱、别隐品碱、血根碱和白屈菜红碱含量；采用高效液相色谱法构建基于不同产地的博落回果化学成分指纹图谱；利用主成分分析和聚类分析方法，分析博落回果中共有指纹峰、产地和药材品质的关联性。结果 构建不同产地博落回果化学成分指纹图谱，确定11个共有指纹峰；主成分分析和聚类分析将8个产地的博落回果分为2大类，其中共有峰5、7、8、9和11对指纹图谱差异贡献较大，6号峰是指纹图谱参照峰的最佳选择。结论 本实验建立的博落回果化学成分指纹图谱具有良好的精密度、重复性和稳定性，能够对不同产地博落回果进行品质评价。

Objective To establish the fingerprint profiling of fruits of Macleaya cordata and study the method for its quality evaluation. Methods The fingerprint profiling of M. cordata fruits from different regions was established using high performance liquid chromatography (HPLC) based on the local standard which was the determination method of the content of protopine, allocryptopine, sanguinarine, and chelerythrine from Changsha of Hunan Province in 2009. The principal component analysis (PCA) and cluster analysis (CA) were performed to explore the correlation among the common fingerprint peaks, origins, and quality of M. cordata fruits. Results Eleven common fingerprint peaks were identified in the fingerprint profiling of chemical constituents of M. cordata fruits from different regions. M. cordata fruits produced from eight areas were classified into two classes by PCA and CA method, and there were five common peaks, including peak 5, 7, 8, 9, and 11 with significant contribution on the regional difference of the fingerprint. Also, common peak 6 was the right peak as the reference peak because of its less variation, appropriate retention time and intensity. Conclusion The fingerprint profiling of chemical constituents of M. cordata fruits established in this study has good precision, repeatability, and stability, which can be used to evaluate the quality of fruits of M. cordata from different producing areas.

国家重点研发计划资助（2017YFD0501500）；国家自然科学基金资助项目（31600238）；湖南省自然科学基金资助项目（2016JJ4040）；湖南省教育厅科学研究项目（16C0769）