目的 通过Plackett-Burman联用Box-Behnken响应面法优化少腹逐瘀凝胶贴膏剂基质处方并对其体外透皮特性进行研究。方法 在预实验基础上，采用Plackett-Burman设计联用Box-Behnken响应面法，以外观性状、初黏力、持黏力和剥离强度为综合指标优化最优处方，采用改良Franz扩散池法，以阿魏酸、芍药苷、异鼠李素-3-O-新橙皮苷、香蒲新苷为评价指标，考察不同用量氮酮促渗剂对凝胶贴膏体外透皮渗透量的影响。结果 凝胶贴膏最优处方为Viscomate^TM NP-700-酒石酸-高岭土（2.42∶0.16∶0.96），3%氮酮对指标成分促渗效果良好，阿魏酸、芍药苷、异鼠李素-3-O-新橙皮苷、香蒲新苷体外透皮速率依次为6.889 4、1.917 4、0.842 0、0.893 7 μg/（cm²·h）。结论 制备的少腹逐瘀凝胶贴膏膏体均匀，敷贴无残留，具有较好的释药性和透皮性，透皮行为符合零级动力学方程。

Objective To optimize the matrix formulation for Shaofu Zhuyu Cataplasm (SZC) by Plackett-Burman design combined with Box-Behnken response surface method and study its transdermal permeation properties in vitro. Methods In this paper, the appearance description, initial bonding strength, endurance bonding strength, and peel strength were taken as indexes. Based on the result of a single factor experiment, the formula for the SZC was optimized by Plackett-Burman combined with Box-Behnken method. Franz diffusing cells method was chosen to investigate the tansdermal infiltration capacity of SZC with different dosage penetration enhancers of azone in vitro, taking ferulic acid, paeoniflorin, isorhamnetin-3-O-neohesperidin, and typhaneoside as index components. Results NP-700, tartaric acid and kaolin had significant effects on the properties of SZC. The optimal ratio of the prescription was as follows:NP-700-tartaric acid-kaolin (2.42:0.16:0.96). The promoting effect of 3% concentration for index component was better, and the transdermal rates of ferulic acid, paeoniflorin, isorhamnetin-3-O-neohesperidin, and typhaneoside were 6.889 4, 1.917 4, 0.852 0, and 0.893 7 μg/(cm²∙h), respectively. Conclusion The SZC was uniform, without residual behavior in application. And it had a good release and transdermal properties, and transdermal actions were consistent with zero-order kinetics.

重庆市科委应用开发重大项目（cstc2014yykfC10006）