目的 分析林下山参制浆后人参皂苷Re、Rg₁、Rb₁、Rg₃、Rh₁、Rh₂含量的变化情况。方法 采用HPLC-UV法，Innoval ODS-2色谱柱（250 mm×4.6 mm，5 μm）；流动相为乙腈-水溶液进行梯度洗脱；柱温30℃；体积流量1.0 mL/min；进样体积20 μL；检测波长203 nm。结果 林下山参中6种人参皂苷Re、Rg₁、Rb₁、Rg₃、Rh₁、Rh₂质量分数分别由制浆前的0.651、0.506、0.363、0.014、0.023、0.031 mg/g变化为制浆后的0.517、0.413、0.105、0.122、0.214、0.098 mg/g。人参皂苷Re、Rg₁、Rb₁、Rg₃、Rh₁、Rh₂分别在2.5～100 mg/L具有良好的线性关系，r²均大于0.999 5；精密度、稳定性及重复性良好；平均加样回收率为95%～105%，RSD为1.25%～3.05%。结论 林下山参中6种人参皂苷Re、Rg₁、Rb₁、Rg₃、Rh₁、Rh₂的含量在制浆前、后发生变化。林下山参制浆后人参皂苷Re、Rg₁、Rb₁的含量降低，稀有人参皂苷Rg₃、Rh₁、Rh₂的含量分别增高8.7、9.3、3.2倍。基于HPLC最佳分离参数建立的6种人参皂苷成分同时分析的方法具有较好的准确性和可靠性，可为林下山参浆的质量评价提供科学依据。

Objective To analyze the content changes of six kinds of ginsenosides Re, Rg₁, Rb₁, Rg₃, Rh₁, and Rh₂ after pulping of mountain cultivated ginseng. Methods The HPLC-UV method was performed on an Innoval ODS-2 chromatographic column (250 mm×4.6 mm, 5 μm), gradient elution of acetonitrile and water with column temperature 30℃, at a flow rate of 1.0 mL/min, and detected at 203 nm with injection volume as 20.0 μL. Results The content of six kinds of ginsenosides Re, Rg₁, Rb₁, Rg₃, Rh₁ and Rh₂ were changed from 0.651, 0.506, 0.363, 0.014, 0.023, 0.031 mg/g to 0.517, 0.413, 0.105, 0.122, 0.214, 0.098 mg/g after pulping of mountain cultivated ginseng. The calibration curve was liner within 2.5-100 mg/L for ginsenoside Re, Rg₁, Rb₁, Rg₃, Rh₁, and Rh₂, respectively, with the correlation r² > 0.999 5 and perfect precision, stability, and repeatability. The average recoveries ranged from 95% to 105%, and RSD values varied from 1.25% to 3.5%. Conclusion The content of six kinds of ginsenosides Re, Rg₁, Rb₁, Rg₃, Rh₁, and Rh₂ in mountain cultivated ginseng were changed after the pulping. The content of ginsenoside Re, Rg₁, and Rb₁ was reduced and rare ginsenoside Rg₃, Rh₁, and Rh₂ was increased by 8.7 times, 9.3 times, and 3.2 times respectively after the pulping. The HPLC method for simultaneous determination of six kinds of ginsenosides has good accuracy and reliability and can provide scientific basis for the quality evaluation of mountain cultivated ginseng pulp.

天然创新药物原料及提取物工业化色谱制备工程研究中心资助项目