目的 建立运用超高效液相色谱-串联四极杆质谱联用技术同时测定西洋参花蕾中4种皂苷类成分（人参皂苷Re、拟人参皂苷F₁₁、人参皂苷Rb₃、人参皂苷Rd）含量的分析方法。方法 采用Waters Acquity UPLC BEH C₁₈（50 mm×2.1 mm，1.7μm）色谱柱；以乙腈和0.05%氨水为流动相进行梯度洗脱；体积流量为0.45 mL/min；柱温35℃；采用电喷雾离子源（ESI）负离子检测方式，多反应监测模式（MRM）。结果 该分析方法的精密度、稳定性、重复性和加样回收率考察结果均符合要求，各对照品在相应的浓度范围内，线性关系良好。结论 建立的方法快速，简便，准确度高，可靠性强，可用于西洋参花蕾中皂苷类成分的分析。

Objective To establish the determination method of four ginsenosides from flower buds of Panax quinquefolium by Waters Acquity UPLC H-Class with Xevo TQD. Methods The chromatographic separation was performed on a Waters Acquity UPLC BEH C₁₈column (50 mm×2.1mm, 1.7 μm). The mobile phase was a mixture of acetonitrile and water containing 0.05% ammonium hydroxide with gradient elution; The flow rate was 0.45 mL/min, and the column temperature was 35℃; Multiple reaction monitoring (MRM) acquisition under negative ion scan mode by ESI was also performed. Results The method was established with good precision, stability, repeatability, and accuracy. Ginsenoside Re, pseudo-ginsenoside F₁₁, ginsenoside Rb₃, and ginsenoside Rd showed a good linearity in the range of corresponding concentrations. Conclusion The UPLC-MS/MS method is rapid, simple, accurate, reliable, which can be used for the analysis of ginsenosides from flower buds of P. quinquefolium.