目的 探究滇重楼对高温抗性适应能力，为滇重楼在中南地区规模化引种栽培提供依据。方法 以滇重楼生长健壮苗为实验材料，在25℃/20℃（昼温/夜温）、33℃/28℃（昼温/夜温）、39℃/34℃（昼温/夜温）的不同温度条件下连续胁迫处理7 d，测定其叶片生理指标，并进行显著性与相关性分析。结果 随着温度的升高与胁迫时间的持续延长，在33℃/28℃与39℃/34℃下处理7 d后，滇重楼叶相对含水量明显下降，分别比对照（25℃/20℃）低17.38%、58.25%；相对电导率与丙二醛（MDA）含量均持续上升，其中39℃/34℃处理下上升幅度最大，电导率比对照高3.59倍，MDA含量比对照高2.55倍；39℃/34℃处理下可溶性糖含量逐渐下降，比对照低33.25%；可溶性蛋白含量均呈波浪状变化，33℃/28℃处理组波动幅度较大，7 d后上升为对照的1.32倍；处理组超氧化物歧化酶（SOD）活性均先升高后下降，39℃/34℃处理组4 d后达到峰值，比对照高2.35倍；处理组过氧化物酶（POD）活性均逐渐上升，39℃/34℃处理组4 d后上升为对照的2.33倍，之后变化幅度逐渐减缓。结论 温度对滇重楼的各项生理指标均有明显的影响，温度为25℃/20℃条件下，植物生长状态良好，为适宜生长温度，33℃/28℃温度条件下，长时间的持续胁迫处理会引起植株叶片损伤，39℃/34℃持续高温胁迫则会严重损伤植株生理活性与形态结构，严重时甚至会导致植株死亡。

Objective To provide a reference for the introduction and cultivation of Paris polyphylla var. yunnanensis in mid-south region of China, its resistance adaptation ability of high temperature was explored. Methods Strong seedlings of P. polyphylla var. yunnanensis were chosen and treated under different temperature conditions (25℃/20℃, 33℃/28℃, 39℃/34℃) for 7 d, and then investigated the effect on their related physiological indexes. Results The results showed that with the increase of temperature and stress time, the relative water content of the leaves of P. polyphylla var. yunnanensis decreased significantly under the treatment of 33℃/28℃ and 39℃/34℃ for 7 d, which was 17.38% and 58.25% lower than the control (25℃/20℃) respectively. The relative conductivity and the content of malondialdehyde (MDA) were both increased continuously, and it was respectively 3.59 times and 2.55 times higher than that of control under the temperature of 39℃/34℃. The content of soluble sugar decreased gradually at 39℃/34℃, which was 33.25% lower than that of the control. The changing of soluble protein content showed a wavy trend, and the fluctuation range of 33℃/28℃ treatment groups was larger than that of the control group, which increased to 1.32 times of the control after 7 d. The SOD increased firstly and then decreased. The peak value was reached after 4 d at 39℃/34℃, which was 2.35 times higher than that of the control group. The POD increased gradually in treatment group, which increased to 2.33 times of that in control group at 39℃/34℃ for 4 d, and the amplitude of the change gradually slowed down. Conclusion In the experimental temperature range, P. polyphylla var. yunnanensis grew well at 25℃/20℃, which was its optimal growth temperature. Long-term continuous temperature stress treatment at 33℃/28℃ can cause damage to plant leaves; High temperature at 39℃/34℃ will seriously damage the physiological and morphological structure of the plant, and even lead to the death of the plant.

湖南省科技计划项目（2016TP2007）；湖南省林业科技计划项目（XLK201440）