目的 观察人参皂苷Rh₂（G-Rh₂）对人胃癌SGC7901/ADR耐药细胞增殖、细胞周期及化疗敏感性的影响。方法 MTT法检测G-Rh₂与阿霉素（ADR）联用对SGC7901/ADR细胞增殖的影响，并计算逆转倍数（RF）；流式细胞术检测G-Rh₂对SGC7901/ADR细胞周期的影响；蛋白印迹法检测G-Rh₂对SGC7901/ADR细胞P-糖蛋白（P-gp）、Bcl-2蛋白表达水平的影响。结果 与ADR单药处理后细胞的半数抑制浓度（IC₅₀）值（54.52 μmol/L）比较，G-Rh₂与ADR联用时SGC7901/ADR细胞IC₅₀值（30.14 μmol/L）明显下降，RF为1.81；G-Rh₂与ADR联用能够将SGC7901/ADR细胞周期阻滞在G₂/M期，与ADR单药处理比较，联用组细胞P-gp、Bcl-2的蛋白表达水平显著降低（P<0.05）。结论 G-Rh₂联合ADR能够提高SGC7901/ADR细胞的化疗敏感性，可能通过阻滞细胞周期、增加细胞凋亡进而抑制细胞增殖。

Objective To observe the effect of ginsenoside Rh₂ (G-Rh₂) on the proliferation, cell cycle and chemotherapy sensitivity of human gastric cancer cell line SGC7901/ADR. Methods MTT assay was used to detected the effects of G-Rh₂ and adriamycin (ADR) on the proliferation of drug-resistant SGC7901/ADR cells and to calculate the reversal fold (RF). Flow cytometry was selected to detect The effects of G-Rh₂ on cell cycle. the effects of G-Rh₂ on the expression of P-gp and Bcl-2 proteins in drug-resistant SGC7901/ADR cells was detected by Western blotting. Results Compared with the IC₅₀ value (54.52 μmol/L) after the ADR single drug treatment, the IC₅₀ value (30.14 μmol/L) of the cells treated with G-Rh₂ and ADR decreased significantly, and RF was 1.81. G-Rh₂ combined with ADR arrested the cell cycle in G₂/M phase and significantly decreased the protein expression of P-gp and Bcl-2(P < 0.05). Conclusion G-Rh₂ combined with ADR could increase the chemosensitivity of SGC7901/ADR cells, which may inhibit the proliferation of gastric cancer cells by blocking cell cycle and increasing apoptosis.

国家自然科学基金重点资助项目（8143000538）；国家自然科学基金面上项目（8177110272）；中国博士后科学基金面上项目（2017M613351）